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PerCP/Cyanine5.5 anti-mouse CD90.1 (Thy1.1) Antibody

Pricing & Availability
Clone
S20007C (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy-1.1, CD90, CD90.1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
S20007C_PerCP-Cyanine5-5_CD90-1_Antibody_042123
FVB mouse splenocytes were stained with anti-mouse CD90.1 (Thy1.1) (clone S20007C) PerCP/Cyanine5.5 (left) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right). Cells were costained with anti-mouse CD3 FITC.
  • S20007C_PerCP-Cyanine5-5_CD90-1_Antibody_042123
    FVB mouse splenocytes were stained with anti-mouse CD90.1 (Thy1.1) (clone S20007C) PerCP/Cyanine5.5 (left) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right). Cells were costained with anti-mouse CD3 FITC.
Compare all formats See PerCP/Cyanine5.5 spectral data
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109003 25 µg 100 CHF
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109004 100 µg 328 CHF
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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Thy1.1 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone S20007C antibody reacts with rat CD90 and mouse CD90.1 (Thy 1.1), which is expressed by AKR/J, PL, and FVB strains, but not CD90.2 strains (C57BL/6, BalB/C, etc).

Clone S20007C partially blocks binding of clone OX-7 on target cells.

RRID
AB_2941383 (BioLegend Cat. No. 109003)
AB_2941383 (BioLegend Cat. No. 109004)

Antigen Details

Structure
Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

Function
Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
Interacts with CD45
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Death, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Campbell DG, et al. 1981. Biochem J. 195:15-30.
  2. Hosseinzadeh H and Goldschneider I. 1993. J Immunol. 150:1670-9.
Gene ID
21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt
View information about CD90.1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 04.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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