PerCP/Cyanine5.5 anti-mouse H-2Kb Antibody anti-H-2Kb

Pricing & Availability

Clone: AF6-88.5 (See other available formats)
Regulatory Status: RUO
Other Names: MHC class I
Isotype: Mouse (BALB/c) IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

AF6-88dot5_PerCPCy55_122209 — C57BL/6 splenocytes stained with AF6-88.5 PerCP/Cyanine5.5

Compare all formats See PerCP/Cyanine5.5 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

116515

25 µg

141 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

116516

100 µg

347 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

The AF6-88.5 antibody reacts with H-2K^b MHC class I alloantigen expressed on nucleated cells from mice of the H-2K^b haplotype. H-2K^b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation² and immunohistochemical staining of acetone-fixed frozen sections³. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
RRID: AB_1967107 (BioLegend Cat. No. 116515)
AB_1967107 (BioLegend Cat. No. 116516)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14972 View all products for this Gene ID
UniProt: View information about H-2Kb on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All H-2Kb Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse H-2K^b	AF6-88.5	FC
FITC anti-mouse H-2K^b	AF6-88.5	FC
PE anti-mouse H-2K^b	AF6-88.5	FC
Purified anti-mouse H-2K^b	AF6-88.5	FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse H-2K^b	AF6-88.5	FC
Alexa Fluor® 647 anti-mouse H-2K^b	AF6-88.5	FC
Pacific Blue™ anti-mouse H-2K^b	AF6-88.5	FC
PerCP/Cyanine5.5 anti-mouse H-2K^b	AF6-88.5	FC
APC anti-mouse H-2K^b	AF6-88.5	FC
PE/Cyanine7 anti-mouse H-2K^b	AF6-88.5	FC
Alexa Fluor® 700 anti-mouse H-2K^b	AF6-88.5	FC
Brilliant Violet 510™ anti-mouse H-2K^b	AF6-88.5	FC
Brilliant Violet 421™ anti-mouse H-2K^b	AF6-88.5	FC

Certificate of Analysis

Customers Also Purchased

FITC anti-mouse CD38

Clone: 90

PE anti-mouse CD170 (Siglec-F)

Clone: S17007L

PE anti-mouse H-2K^b bound to SIINFEKL

Clone: 25-D1.16

PE/Cyanine7 anti-mouse CD154 (CD40L)

Clone: SA047C3

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.