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PerCP/Cyanine5.5 anti-mouse I-Ab Antibody

Pricing & Availability
Clone
AF6-120.1 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Mouse (BALB/c) IgG2a, κ
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Product Citations
publications
AF6-120dot1_PerCPCyanine55_I-Ab_Antibody_101818
C57BL/6 mouse splenocytes were stained I-Ab (clone AF6-120.1) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • AF6-120dot1_PerCPCyanine55_I-Ab_Antibody_101818
    C57BL/6 mouse splenocytes were stained I-Ab (clone AF6-120.1) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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116415 25 µg 141 CHF
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116416 100 µg 347 CHF
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Description

The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10J splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Does not react with other haplotypes (e.g., d, f, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
  2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
  3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
  4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
  5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
  6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
  7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
  8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
  9. Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
Product Citations
  1. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  2. Kumar Das D, et al. 2022. J Biol Chem. 298:102596. PubMed
  3. Pfeifhofer-Obermair C, et al. 2022. Bio Protoc. 12:e4378. PubMed
  4. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  5. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  6. Wong E, et al. 2019. Cell Rep. 29:3047. PubMed
  7. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  8. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  9. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  10. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  11. Uto-Konomi A, et al. 2012. PLoS One. 7:e40343. PubMed
  12. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  13. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  14. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  15. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_1953308 (BioLegend Cat. No. 116415)
AB_1953308 (BioLegend Cat. No. 116416)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ab on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10.18.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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