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PerCP/Cyanine5.5 anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B10_PerCPCyanine55_T-Bet_Antibody_1_012519
Human peripheral blood lymphocytes were surface stained with CD3 FITC, treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), then stained with T-bet (clone 4B10) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isoty
  • 4B10_PerCPCyanine55_T-Bet_Antibody_1_012519
    Human peripheral blood lymphocytes were surface stained with CD3 FITC, treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), then stained with T-bet (clone 4B10) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isoty
Compare all formats See PerCP/Cyanine5.5 spectral data
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644805 25 µg 200 CHF
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644806 100 µg 411 CHF
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  2. Glaubitz J, et al. 2023. Gut. :. PubMed
  3. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  4. Zander R, et al. 2022. Immunity. 55:475. PubMed
  5. Read KA, et al. 2023. Nat Commun. 14:1652. PubMed
  6. Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed
  7. Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed
  8. Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005817. PubMed
  9. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  10. Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed
  11. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  12. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  13. Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed
  14. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  15. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  16. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  17. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  18. Hurton L, et al. 2016. Proc Natl Acad Sci U S A. 113: E7788 - E7797. PubMed
  19. Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed
  20. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  21. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  22. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  23. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  24. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
RRID
AB_1595488 (BioLegend Cat. No. 644805)
AB_1595488 (BioLegend Cat. No. 644806)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 5    Revision Date: 01.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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