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PerCP/Cyanine5.5 Streptavidin

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Regulatory Status
RUO
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Product Citations
publications
Steptavadin_PerCpCyanine55_101818
Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-PerCP/Cyanine5.5 (filled histogram), or directly stained with SAV-PerCP/Cyanine5.5 (open histogram).
  • Steptavadin_PerCpCyanine55_101818
    Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-PerCP/Cyanine5.5 (filled histogram), or directly stained with SAV-PerCP/Cyanine5.5 (open histogram).
See PerCP/Cyanine5.5 spectral data
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405214 100 µg 293 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP/Cyanine5.5 is useful for detecting biotinylated antibodies. The excitation of PerCP/Cyanine5.5 by 488 nm laser light induces a light emission maximum of 690 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PerCP/Cyanine5.5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-PerCP/Cyanine5.5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP/Cyanine5.5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-PerCP/Cyanine5.5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP/Cyanine5.5 is 110 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Killeen ME, et al. 2013. J. Immunol. 190:4324. PubMed
  2. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
  3. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
Product Citations
  1. Li X, et al. 2022. Haematologica. 107:2576. PubMed
  2. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  3. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  4. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  5. Feng X, et al. 2023. Nat Commun. 14:3208. PubMed
  6. Zakeri A, et al. 2021. J Extracell Vesicles. 10:e12131. PubMed
  7. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
  8. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  9. Pease NA, et al. 2021. Cell Reports. 34(12):108888. PubMed
  10. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  11. Ng KK et al. 2018. eLife. 7 pii: e37851. PubMed
  12. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  13. Dong S, et al. 2021. Nature. 591:117. PubMed
  14. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  15. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  16. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  17. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  18. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  19. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  20. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  21. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  22. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  23. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  24. Lopes N, et al. 2022. Elife. 11:. PubMed
  25. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  26. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  27. Killeen M, et al. 2013. J Immunol. 190:4324. PubMed
  28. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  29. Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed
  30. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
RRID
AB_2716577 (BioLegend Cat. No. 405214)

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 5    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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