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PerCP Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-PerCP
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Product Citations
publications
See PerCP spectral data
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405213 100 µg 265 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP is useful for detecting biotinylated antibodies. The excitation of PerCP by 488 nm laser light induces a light emission maximum of 675 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PerCP solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-PerCP is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP is 120 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Mara-Koosham G, et al. 2011. Infect Immun. 79:1770. PubMed
  2. Liao G, et al. 2012. Gastroenterology. 142:582. PubMed
  3. Smith KA, et al. 2012. Infect Immun. 80:3481. PubMed
  4. Li J, et al. 2013. Am J respir Cell Mol Biol. 48:314. PubMed
  5. Michael A, et al. 2013. J. Immunol. 190:5534. PubMed
  6. Tsiganov EN, et al. 2014. J Immunol. 192:4718. PubMed
  7. Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Screnci B, et al. 2022. iScience. 25:105665. PubMed
  3. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  4. González-Tajuelo R, et al. 2017. Sci Rep. 7:41841. PubMed
  5. Li J, et al. 2013. Am J Respir Cell Mol Biol. 48:314. PubMed
  6. Jones M, et al. 2016. PLoS One. 11: 0157271. PubMed
  7. Mara-Koosham G, et al. 2011. Infect Immun. 79:1770. PubMed
  8. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  9. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  10. Tsiganov E, et al. 2014. J Immunol. 192:4718. PubMed
  11. Köchl R, et al. 2020. Elife. 9:00. PubMed
  12. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  13. Liao G, et al. 2012. Gastroenterology. 142:582. PubMed
  14. Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed
  15. Klein H, et al. 2016. J Neuropathol Exp Neurol. 75: 1031 - 1047. PubMed
  16. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  17. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  18. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  19. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
  20. Smith K, et al. 2012. Infect Immun . 80:3481. PubMed
  21. Seong J, et al. 2018. Development. 145:14. PubMed

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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