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PerCP/Cyanine5.5 anti-human CD16

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone
3G8
Workshop
V NK80
Other Names
FcγRIII
Isotype
Mouse IgG1, κ
3G8_PerCPCyanine5-5_CD16_ASR_042823
Typical results from human peripheral blood lymphocytes stained either with 3G8 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram)
  • 3G8_PerCPCyanine5-5_CD16_ASR_042823
    Typical results from human peripheral blood lymphocytes stained either with 3G8 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram)
Compare all formats See PerCP/Cyanine5.5 spectral data
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980118 500 µL 588 CHF
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
200 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

Suggested for Flow Cytometry
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
  6. Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References
  1. Fleit H, et al. 1982. Proc Natl Acad Sci U S A. 79:3275-9.
  2. Stroncek D, et al. 1991. Blood. 77:1572-80.
  3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381-90.
Go To Top Version: 1    Revision Date: 05.08.2023

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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