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Precision Count Beads™

Pricing & Availability
Regulatory Status
RUO
Other Names
Counting Beads
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Product Citations
publications
Precision_Count_Beads_1_051916
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail using a lyse, no-wash method. Precision Count Beads™ were added and bead count and CD4+CD3+ cell count was determined by gating on beads and cells as shown, gated on CD45+ lymphocytes. The number of total cells is determined as described in the protocol.
  • Precision_Count_Beads_1_051916
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail using a lyse, no-wash method. Precision Count Beads™ were added and bead count and CD4+CD3+ cell count was determined by gating on beads and cells as shown, gated on CD45+ lymphocytes. The number of total cells is determined as described in the protocol.
  • Precision_Count_Beads_2_051916
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424902 100 tests 258 CHF
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Description

Precision Count Beads™ can be used to obtain absolute counts of cells or any other samples acquired on a flow cytometer.  Precision Count Beads™  are fluorescent particles about 10µm that can be detected across a broad range of wavelengths (405-635nm excitation and 400-800nm emission).  Absolute numbers of cells can be determined by comparing the bead count and cell count.  Precision Count Beads™ can work with all kinds of assays including a lyse, no-wash whole blood assay.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Precision Count Beads™ are supplied in a solution containing 0.02% sodium azide and 0.01% NP40.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Vigorously vortex the Precision Count Beads™ bottle for 30-40 seconds to ensure complete mixing and break up of aggregates that may occur during storage. For flow cytometric analysis, the suggested use of this reagent is 100 µl per test. Please read the Precision Count Beads™ protocol and applications for further instructions.

Application Notes

Precision Count Beads™ are designed for counting the absolute number of cells in a complex population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm).

Product Citations
  1. Natoli M, et al. 2021. Front Oncol. 644608:11. PubMed
  2. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  3. Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed
  4. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 05.19.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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