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Purifed anti-SHC1 Antibody

Pricing & Availability
Clone
O91H8 (See other available formats)
Regulatory Status
RUO
Other Names
SHC Adaptor Protein 1, SHC-Transforming Protein 1, SHC-Transforming Protein 3, SHC-Transforming Protein A, SH2 Domain Protein C1, SHCA, SHC, SHC (Src Homology 2 Domain Containing) Transforming Protein 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
/O91H8_PURE_SHC1_Antibody_1_072919
Whole cell extracts (15 µg protein) from HeLa cells transfected with non-targeting control siRNA (siCON) or siRNA targeting SHC1 (siSHC1) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SHC1 Antibody, clone O91H8, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • /O91H8_PURE_SHC1_Antibody_1_072919
    Whole cell extracts (15 µg protein) from HeLa cells transfected with non-targeting control siRNA (siCON) or siRNA targeting SHC1 (siSHC1) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SHC1 Antibody, clone O91H8, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • /O91H8_PURE_SHC1_Antibody_2_072919
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SHC1 Antibody, clone O91H8, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. *: Non-specific band
  • /O91H8_PURE_SHC1_Antibody_3_072919
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Mouse IgG1, κ Isotype Ctrl Antibody (Cat. No. 401402) or Purified anti-SHC1 Antibody, clone O91H8. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SHC1 Antibody, clone O91H8. Lane M: Molecular Weight marker.
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669701 25 µg 101 CHF
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669702 100 µg 253 CHF
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Description

SHC1 is a ubiquitously expressed adaptor protein involved in receptor tyrosine kinase signaling including the activation of the RAS/Raf/MAPK pathway. Three isoforms of SHC1 (p66, p52, p46) have been described, each with unique cellular functions. SHC1 has an SH2 domain and a protein tyrosine phosphate binding (PTB) domain, which recognize activated receptor tyrosine kinases. Tyrosine residues located in the CH1 domain of SHC1 are phosphorylated by tyrosine kinases and serve as docking sites for proteins such as Grb2. SHC1-bound Grb2 recruits SOS, which activates Ras, thereby promoting cell proliferation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human SHC1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, KO/KD-WB - Verified
ICC, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814514 (BioLegend Cat. No. 669701)
AB_2814514 (BioLegend Cat. No. 669702)

Antigen Details

Structure
SHC1 is a 583 amino acid protein with a predicted molecular weight of 66 kD.
Distribution

Cytosol/Ubiquitously expressed

Function
Adaptor protein
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Adaptor Proteins
Antigen References
  1. Zheng Y, et al. 2013. Nature. 499:166
  2. Ravichandran K, et al. 2001. Oncogene. 20:6322
  3. Migliaccio E, et al. 1999. Nature. 402:309
Gene ID
6464 View all products for this Gene ID
UniProt
View information about SHC1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.29.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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