- Clone
- A18010A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eukaryotic Translation Initiation Factor 4E Binding Protein 1, Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1, PHAS-I
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
619601 | 25 µg | 112 CHF | ||||
619602 | 100 µg | 265 CHF |
In eukaryotes, the mTOR kinase signaling pathway links cell growth and proliferation with nutrient availability. Under nutrient-deplete conditions, eukaryotic translation initiations factor 4E-binding protein 1 (4EBP1) binds the general translation factor eIF4E, resulting in an attenuation of cap-dependent translation. Under nutrient-replete conditions, mTORC1 and mTORC2 complexes phosphorylate 4EBP1 at Threonine 70 and disrupt the formation of the 4EBP1-eIF4E complex, thereby alleviating the repressive function of 4EBP1.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human 4EBP1 phosphorylated at Threonine 70
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone has not been tested for ICC and IP.
- RRID
-
AB_2814468 (BioLegend Cat. No. 619601)
AB_2814468 (BioLegend Cat. No. 619602)
Antigen Details
- Structure
- 4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD.
- Distribution
-
Cytosol and nucleus/ubiquitously expressed
- Function
- Regulation of global translation
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Brunn GJ, et al. 1997. Nature. 277:99.
- Hara K, et al. 2002. Cell. 110:177.
- Ekim B, et al. 2011. Mol. Cell Biol. 31:2787.
- Gene ID
- 1978 View all products for this Gene ID
- UniProt
- View information about 4EBP1 on UniProt.org
Other Formats
View All 4EBP1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-4EBP1 Phospho (Thr70) | A18010A | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-4EBP1 Phospho (Thr70)
Whole cell extracts (15 µg protein) from untreated (-) or LY...
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