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Purified anti-ABL1 Phospho (Tyr245) Antibody

Pricing & Availability
Clone
A21014A (See other available formats)
Regulatory Status
RUO
Other Names
ABL Proto-Oncogene 1 Nonreceptor tyrosine kinase; P150; C-ABL; Aberson Tyrosine-Protein kinase 1; ABL; CHDSKM
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
a. A21014A_PURE_ABL1-Phospho_WB_100523
Whole cell extracts (15 µg total protein) from K562 cells untreated or treated with a Pan-Bcr-Abl inhibitor (AP 24534) (50 nM, 4 hours), were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-ABL1 Phospho (Tyr245) (clone A21014A) (left), and anti-Pan ABL1 (right) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • a. A21014A_PURE_ABL1-Phospho_WB_100523
    Whole cell extracts (15 µg total protein) from K562 cells untreated or treated with a Pan-Bcr-Abl inhibitor (AP 24534) (50 nM, 4 hours), were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-ABL1 Phospho (Tyr245) (clone A21014A) (left), and anti-Pan ABL1 (right) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • b. A21014A_PURE_ABL1-Phospho_ICFC_052523
    K562 cells untreated (positive control, filled histogram), or treated with a Pan-BCR-ABL inhibitor (AP24534) (negative control, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-ABL1 Phospho (Tyr245) (clone A21014A), or purified mouse IgG2b, κ isotype control (Cat. No. 400301) (open histogram, dashed line), followed by PE goat anti-mouse IgG (Cat. No. 405307).
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618651 25 µg 176 CHF
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618652 100 µg 440 CHF
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Description

ABL1 is a protooncogene that encodes a tyrosine kinase protein. ABL1 is involved in multiple cellular processes such as adhesion, cell division, autophagy, differentiation and response to stress. ABL1 kinase activity is negatively regulated by interaction with its SH3 domain, thus deletion of the SH3 domain results in an oncogene. Chromosomal translocation involving ABL1 has been found in various leukemias, where the most notable is the t (9;22) that results in a fusion with the breakpoint cluster region gene (BCR). This rearrangement is present in many cases of chronic myelogenous leukemia, and leads to an unregulated tyrosine kinase activity which induces uncontrolled cellular proliferation. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human ABL1 phosphorylated at Tyr245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICFC, cells were fixed and permeabilizes using Cyto-Fast™ Buffer system. 

RRID
AB_3068060 (BioLegend Cat. No. 618651)
AB_3068060 (BioLegend Cat. No. 618652)

Antigen Details

Structure
ABL1 is an approximately 125 kD protein. However, when it is fused with BCR, the molecular weight is around 210 kD.
Distribution

Mainly located in cytoplasm, but it can also be found in the nucleus, mitochondria and endoplasmic reticulum

Function
ABL1 is a protein kinase that is negatively regulated via interaction with its SH3 domain, and after deletion of the SH3 domain, it becomes an oncogene. It also plays a role in several cellular processes like: cell growth, cell motility, adhesion, autophagy, and apoptosis.
Interaction
Interacts with the network of fibers called the actin cytoskeleton
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family
Phospho-Proteins
Antigen References
  1. Burslem GM, et al. 2019. Cancer Res. 79:4744-4753.
  2. Piedimonte M, et al. 2019. BMC Cancer. 19:50.
  3. Wylie AA, et al. 2017. Nature. 543:733-737. 
Gene ID
25 View all products for this Gene ID
UniProt
View information about ABL1 Phospho (Tyr245) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05.25.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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