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Purified anti-ADH1 Antibody

Pricing & Availability
Clone
Poly28635 (See other available formats)
Regulatory Status
RUO
Other Names
ADH1, Alcohol Dehydrogenase 1C (Class I), Gamma Polypeptide, Alcohol Dehydrogenase Subunit Gamma, Aldehyde Reductase, EC 1.1.1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28635_PURE_ADH1_Antibody_1_102319
Western blot of purified anti-ADH1 antibody (Poly28635). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 10 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28635_PURE_ADH1_Antibody_1_102319
    Western blot of purified anti-ADH1 antibody (Poly28635). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 10 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28635_PURE_ADH1_Antibody_2_102319
    IHC staining of purified anti-ADH1 antibody (Poly28635) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28635_PURE_ADH1_Antibody_3_102319
    IHC staining of purified anti-ADH1 antibody (Poly28635) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28635_PURE_ADH1_Antibody_4_102319
    IHC staining of purified anti-ADH1 antibody (Poly28635) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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863501 25 µg 125 CHF
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Description

ADH1 is the γ subunit of class I alcohol dehydrogenase. The alcohol dedydrogenase family is involved in metabolization of a variety of substrates, including ethanol and lipid peroxidation products. ADH1 is related to the signaling pathway of cytochrome P450 metabolism. It is also associated with several diseases including late-onset Parkinson Disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Alcohol Dehydrogenase 1 (ADH1) expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage, aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820148 (BioLegend Cat. No. 863501)

Antigen Details

Structure
ADH1 is a 375 amino acid protein with an apparent molecular mass of ~39 kD.
Distribution

Tissue Distribution: Liver, kidney, pancreas, and gallbladder
Cellular Distribution: Cytosol and nucleus

Function
ADH1 is involved in ethanol and hydroxysteroids metabolism.
Interaction
Ethanol, retinol, hydroxysteroids
Cell Type
Mesenchymal cells
Biology Area
Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
Aldehyde Dehydrogenases
Antigen References
  1. Lieber CS. 2004. Alcohol. 34(1):9-19.
  2. Zuo L, et al. 2013. Hum Genet. 132(7):735-43.
  3. Dumont S, et al. 2018. PLoS One. 13(9):e0204530.
Gene ID
126 View all products for this Gene ID 11522 View all products for this Gene ID 24172 View all products for this Gene ID
UniProt
View information about ADH1 on UniProt.org
Go To Top Version: 1    Revision Date: 10.23.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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