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Purified anti-AKT2 Antibody

Pricing & Availability
Clone
P81B6 (See other available formats)
Regulatory Status
RUO
Other Names
V-Akt Murine Thymoma Viral Oncogene Homolog 2, Protein Kinase B Beta, Rac Protein Kinase Beta, RAC-PK-Beta, PKB Beta, PRKBB, PKBB, HIHGHH, PKBBETA, RAC-BETA
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
P81B6_PURE_AKT2_Antibody_WB_092915
Western blot analysis of cell lysates from HeLa (lane 1), Jurkat (lane 2), and NIH3T3 (lane 3). Purified anti-AKT2 antibody (clone P81B6 ) was used and then was followed by HRP anti-mouse IgG antibody. β-actin antibody (clone poly6221) was used as a loading control.
  • P81B6_PURE_AKT2_Antibody_WB_092915
    Western blot analysis of cell lysates from HeLa (lane 1), Jurkat (lane 2), and NIH3T3 (lane 3). Purified anti-AKT2 antibody (clone P81B6 ) was used and then was followed by HRP anti-mouse IgG antibody. β-actin antibody (clone poly6221) was used as a loading control.
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680202 100 µg 265 CHF
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Description

AKT (Protein kinase B, PKB) is a serine/threonine kinase that plays a key in regulating cell survival, insulin signaling, angiogenesis, and tumor formation. There are three isoforms of AKT- AKT1, 2, and 3, which are also known as PKBα, β, and γ). Increasing evidence suggests that AKT1, 2, and 3 plays an unique role in breast cancer initiation and progression. AKT2 is amplified or overexpressed in cancer with a higher frequency than those found with AKT1. AKT2 is also closely linked to Type II diabetes and is a pivotal player in the Phosphatidylinositol 3 kinase/Akt/Mammalian target of rapamycin (PI3K/mTOR) signaling axis. The deregulation of AKT2 has been associated with acquisition of stem cell-like properties, responsible for invasiveness and tumor progression, metastatic spread, and chemoresistance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment (a.a. 2-149) of human AKT2 expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Crouch MJ, et al. 2019. J Leukoc Biol. 241:106. PubMed
RRID
AB_2566344 (BioLegend Cat. No. 680202)

Antigen Details

Structure
481 amino acids with a predicted molecular weight of approximately 56 kD.
Distribution

Cytoplasm, nucleus, cell membrane, and peripheral membrane protein.

Function
AKT2 plays a ket role in regulating cell survival, insulin, signaling, angiogenesis, and tumor formation.
Interaction
BTBD10, KCTD20, MTCP1, TCL1A, TCL1B, CLK2, PBH2, TRAF6, and CLIP3.
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Pereira L, et al. 2015. Drug Discov. Today 9:1152-8.
2. Yu H, et al. 2015. Vascul. Pharmacol. 71:57-64.
3. Cohen MM Jr. 2013. Am J. Med Genet A. 12:2931-7.
4. Dillon RL, Muller WJ. 2010. Cancer Res. 11:4260-4.
5. Song G, et al. 2005. J. Cell Mol. Med. 1:59-71.

Gene ID
208 View all products for this Gene ID
UniProt
View information about AKT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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