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Purified anti-Angiopoietin-2 Antibody

Pricing & Availability
Clone
M5203F01 (See other available formats)
Regulatory Status
RUO
Other Names
ANG-2, ANGPT2, AGPT2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
M5203F01_Purified_Angiopoietin2_Antibody_WB_122215
Recombinant human angiopoietin-2 (50 ng, lane 1) and HUVEC cell lysate (30 µg, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Angiopoietin-2 (clone M5203F01) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Exposure time was ten seconds for lane 1 and five minutes for lane 2.
  • M5203F01_Purified_Angiopoietin2_Antibody_WB_122215
    Recombinant human angiopoietin-2 (50 ng, lane 1) and HUVEC cell lysate (30 µg, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Angiopoietin-2 (clone M5203F01) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Exposure time was ten seconds for lane 1 and five minutes for lane 2.
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682702 100 µg 265 CHF
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Description

Angiopoietin-2, also known as ANGPT2, belongs to a family of vascular growth factors. It plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of Angiopoietin-2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both Angiopoietin-1 and Angiopoietin-2 are ligands for the receptor tyrosine kinase, Tie-2. Angiopoietin-2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while Angiopoietin-1 predominantly exists as multimers. It has been shown that Angiopoietin-2 is able to promote vascular leakage and neutrophil migration. It can also sensitize TNF-α-induced monocyte adhesion. Thus, it is considered a proinflammatory factor. Although Angiopoietin-2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of Angiopoietin-2 activate the same survival signaling pathway as Angiopoietin-1 to prevent endothelial cell apoptosis via Tie-2/Akt. Additionally, Angiopoietin-2 functions as an agonist in the absence of Angiopoietin-1, but it acts as an antagonist in its presence. Human Angiopoietin-2 shares an 86% amino acid sequence homology with its murine counterpart.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Angiopoietin-2, amino acids (Tyr19-Phe496) (Accession# NP_001138), was expressed with a C-terminal His tag in CHO cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566672 (BioLegend Cat. No. 682702)

Antigen Details

Structure
Oligomer.
Distribution

Secreted protein, low abundance in nucleus. Placenta, uterus, ovaries, and vascular endothelial cells.

Function
Angiopoietin-2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
Interaction
Monocytes and endothelial cells.
Ligand/Receptor
Tie-2.
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Augustin HG, et al. 2009. Nat. Rev. Mol. Biol. 10:165.
2. Ward NL, Dumont DJ. 2002. Semin. Cell Dev. Biol. 1:19-27.
3. Roviezzo F, et al. 2005. J. Pharmacol. Exp. Ther. 314:738.
4. Sturn DH, et al. 2005. Microcirculation 12:393.
5. Murdoch C, et al. 2007. J. Immunol. 178:7405.
6. Yuan HT, et al. 2009. Mol. Cell Biol. 29:2011.
7. Procopio WN, et al. 1999. J. Biol. Chem. 274:30196.
8. Bhandari V, et al. 2006. Nature Med. 12:1286.

Gene ID
285 View all products for this Gene ID
UniProt
View information about Angiopoietin-2 on UniProt.org
Go To Top Version: 1    Revision Date: 12.22.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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