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Purified anti-ATIC Antibody

Pricing & Availability
Clone
F38P7H9 (See other available formats)
Regulatory Status
RUO
Other Names
PURH, IMPCHASE/AICARFT, Bifunctional purine biosynthesis protein PURH
Isotype
Mouse IgG1, κ
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Product Citations
publications
ATIC_WB
Total lysates (15 µg protein) from 293T and 293T/ATIC CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-ATIC antibody, clone F38P7H9 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • ATIC_WB
    Total lysates (15 µg protein) from 293T and 293T/ATIC CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-ATIC antibody, clone F38P7H9 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
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635602 100 µg 229 CHF
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Description

ATIC (AICAR transformylase/IMP cyclohydrolase) belongs to the purH family, expressed in the cytoplasm, predicted molecular weight approximately 65 kD. ATIC catalyzes the penultimate and final steps in the de novo purine nucleotide biosynthesis pathway. ATIC acts as a bifunctional enzyme catalyze the formation of FAICAR and IMP. AICA-ribosiduria is a recently discovered inherited metabolic disease caused by a defect in final steps of purine de novo biosynthesis. Clone F38P7H9 has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat, frog and fruit fly ATIC.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Rat, Frog, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide AHTNLRLFHH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2227799 (BioLegend Cat. No. 635602)

Antigen Details

Structure
Belongs to the purH family, the IMP cyclohydrolase activity resides in the N-terminal region. Predicted molecular weight approximately 65 kD.
Distribution

Cytoplasm

Function
ATIC catalyzes the penultimate and final steps in the de novo purine biosynthesis pathway. ATIC acts as a bifunctional enzyme catalyze the formation of FAICAR and IMP.
Interaction
CDK2, Stomatin, JAK1, NM23B, IKKE, HNRNP, CIA30
Biology Area
Cell Biology
Antigen References

1. Rayl EA, et al. 1996. J. Biol. Chem. 271:2225

Gene ID
471 View all products for this Gene ID
UniProt
View information about ATIC on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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