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Purified anti-ATM Antibody

Pricing & Availability
Clone
MAT3-4G10/8 (See other available formats)
Regulatory Status
RUO
Other Names
ATM Serine/Threonine Kinase, Ataxia Telangiectasia Mutated, Serine-Protein Kinase ATM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MAT3-4G10slash8_PURE_ATM_Antibody_1_091120.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 3-8% Tris-Acetate gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ATM antibody (clone MAT3-4G10/8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • MAT3-4G10slash8_PURE_ATM_Antibody_1_091120.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 3-8% Tris-Acetate gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ATM antibody (clone MAT3-4G10/8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • MAT3-4G10slash8_PURE_ATM_Antibody_2_091120.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 401402) or purified anti-ATM antibody (clone MAT3-4G10/8). The resulting IP fractions and whole cell extract input (6%) were resolved by 3-8% Tris-Acetate gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody raised against a separate epitope of ATM. Lane M: Molecular weight marker.
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939301 25 µg 101 CHF
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939302 100 µg 253 CHF
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Description

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that plays a critical role in cell cycle checkpoints and DNA repair. ATM is normally maintained as an inactive dimer in unstressed cells. Cellular irradiation and other genotoxic events that lead to double-stranded DNA breaks induce a rapid intermolecular autophosphorylation of serine 1981 that results in dimer dissociation and initiation of intrinsic kinase activity. ATM serine 1981 phosphorylation is considered a powerful surrogate of both ATM activation and double-stranded DNA break repair. ATM directly regulates a broad range of substrate proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair, including tumor suppressor proteins p53 and BRCA1, and the checkpoint kinase Chk2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic mouse ATM peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was not tested for ICC due to the detection of proteins of unknown origin by western blot.

RRID
AB_2876766 (BioLegend Cat. No. 939301)
AB_2876766 (BioLegend Cat. No. 939302)

Antigen Details

Structure
ATM is 3056 amino acids protein with a predicted molecular weight of ~351 kD.
Distribution

Ubiquitously expressed, nuclear

Function
Serine, threonine kinase, DNA repair and checkpoint signaling
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Antigen References

1. Lee JH and Paull TT. 2007. Oncogene. 26:7741.
2. Bakkenist CJ and Kastan MB. 2003. Nature. 421:499. 
3. McConville CM, et al. 1996. Am. J. Hum. Genet. 59:320. 
4. Tang X, et al. 2008. Mol. Cell Biol. 28:2559.

Gene ID
472 View all products for this Gene ID
UniProt
View information about ATM on UniProt.org
Go To Top Version: 1    Revision Date: 09.11.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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