- Clone
- W21141A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BRAF, V-Raf Murine Sarcoma Viral Oncogene Homolog B1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, Proto-oncogene B-Raf, BRAF1, BRAF-1, RAFB1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W21141A_PURE_B-Raf_Antibody_1_010824 W21141A_PURE_B-Raf_Antibody_1_010824](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W21141A_PURE_B-Raf_Antibody_1_010824.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-B-Raf (clone W21141A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # | Size | Price | Quantity Check Availability | Save | ||
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625051 | 25 µg | 180 CHF | ||||
625052 | 100 µg | 445 CHF |
B-Raf is one of three proteins in the RAF family of serine/threonine protein kinases. B-Raf regulates the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. Like the other two RAF kinases, A-Raf and C-Raf, B-Raf relays extracellular signals to the nucleus through the MAPK/ERK pathway and regulates cell division, differentiation, and secretion. RAF proteins share similar protein domains and motifs, including multiple protein binding domains such as Ras, lipid, and the 14-3-3 binding motif. RAF proteins mainly reside in the cytoplasm in an inactive state by binding to 14-3-3 proteins. RAFs translocate to the membrane compartment by activation and binding with RAS proteins. Similar to other RAF kinases, B-Raf phosphorylates MAP2K1 (MEK) proteins to activate the MAPK/ERK pathway. Mutations in the gene which encodes B-Raf are highly correlated with inherited cardiofaciocautaneous syndrome and various types of cancers. The B-Raf V600E mutation results in a constitutively active kinase leading to aberrant cell proliferation and invasion. Such mutations are linked to hairy cell leukemia, melanoma, colon cancer, and non-small-cell lung cancer. B-Raf inhibitors are considered as anticancer targets for metastatic melanoma, kidney cancer, and liver cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human B-Raf
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product cross-reacts with mouse in western blotting (WB).
This product is not recommended for immunocytochemistry (ICC). - RRID
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AB_3097499 (BioLegend Cat. No. 625051)
AB_3097499 (BioLegend Cat. No. 625052)
Antigen Details
- Structure
- It forms homodimers and heterodimers with C-Raf. It binds to 14-3-3, Ras, or MAP2K1.
- Distribution
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Cytoplasm, membrane, and nucleus
- Function
- Phosphorylates MAP2K1 and activates the MAPK/ERK pathway
- Interaction
- Regulates the MAPK and ERK pathways; interacts with PFKFB2
- Biology Area
- Cancer Biomarkers, Cardiovascular Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
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- Davies H, et al. 2002. Nature. 17(6892):949-54.
- Wajapeyee N, et al. 2008. Cell. 132:363-74.
- Tiacci E, et al. 2011. N Engl J Med. 364:2305-15.
- Dankner M, et al. 2018. Oncogene. 37:3183-3199.
- Gene ID
- 673 View all products for this Gene ID
- UniProt
- View information about B-Raf on UniProt.org
Other Formats
View All B-Raf Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-B-Raf | W21141A | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-B-Raf
Whole cell extracts (15 µg total protein) from indicated cel...
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