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Purified anti-BCAT1 Antibody

Pricing & Availability
Clone
W18024A (See other available formats)
Regulatory Status
RUO
Other Names
Branched Chain Amino Acid Transanimase 1, BCT1, BCATC, PNAS121, Protein ECA39
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18024A_PURE_BCAT1_Antibody_091120.png
Whole cell lysates (15 µg) from NTERA-2, Daudi, and indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-BCAT1 antibody (clone W18024A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of descending BCAT1 expression levels source (Human Protein Atlas).
  • W18024A_PURE_BCAT1_Antibody_091120.png
    Whole cell lysates (15 µg) from NTERA-2, Daudi, and indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-BCAT1 antibody (clone W18024A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of descending BCAT1 expression levels source (Human Protein Atlas).
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938001 25 µg 101 CHF
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938002 100 µg 253 CHF
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Description

BCAT1 is an aminotransferase that catalyzes the catabolism of the branched-chain amino acids leucine, isoleucine, and valine, making it an important regulator of the TCA cycle. BCAT1 also plays a role in cell cycle progression, differentiation, and apoptosis. Clinical disorders involved with mutations in the BCAT1 gene include hypervalinemia and hyperleucine-isoleucinemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human BCAT1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed to immunoprecipitate BCAT1 from NTERA-2 lysates.

This clone was tested for immunocytochemistry on NTERA-2 cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in dim staining of BCAT1. We therefore do not recommend the clone for this application.

RRID
AB_2876757 (BioLegend Cat. No. 938001)
AB_2876757 (BioLegend Cat. No. 938002)

Antigen Details

Structure
BCAT1 is a 386 amino acid protein with a predicted molecular weight of 43 kD.
Distribution

Ubiquitously expressed/Cytosolic

Function
Aminotransferase enzyme, amino acid catabolism
Cell Type
Macrophages
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References
  1. Papathanassiu AE, et al. 2017. Nat. Commun. 8:16040.
  2. Xu M, et al. 2016. Oncol Lett. 12:2648-2656.
  3. Zhou W, et al. 2013. Mol Cancer. 12:53.
  4. Wegermann K, et al. 2018. PLoS One. 13:e0204308.
  5. Hattori A, et al. 2017. Nature. 545:500-504.
Gene ID
586 View all products for this Gene ID
UniProt
View information about BCAT1 on UniProt.org
Go To Top Version: 1    Revision Date: 09.11.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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