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Purified anti-Beclin-1 Antibody

Pricing & Availability
Clone
O93F3 (See other available formats)
Regulatory Status
RUO
Other Names
ATG6 Autophagy Related 6 Homolog, VSP30
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O93F3_PURE_Beclin-1_Antibody_020720.png
Western blot of purified anti-Beclin-1 antibody (clone O93F3). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O93F3_PURE_Beclin-1_Antibody_020720.png
    Western blot of purified anti-Beclin-1 antibody (clone O93F3). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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849701 25 µg 118 CHF
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849702 100 µg 288 CHF
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Description

Beclin-1 plays a central role in autophagy and is involved in autophagosome formation. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Beclin-1 is necessary for the initiation of autophagy, and it has been shown to be reduced in the brains of patients with Alzheimer’s disease. Reduced Beclin-1 levels lead to decreased autophagy, the accumulation of Aβ peptides, and neurodegeneration in mouse models of Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human Beclin-1 protein (NP_003757) produced in HEK293T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Deng X, et al. 2017. Biochim. Biophys. Acta. 1862(3):337-350 (WB).
Product Citations
  1. Caddeo C, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Rinaldi R, et al. 2021. Cells. 10:. PubMed
RRID
AB_2687306 (BioLegend Cat. No. 849701)
AB_2687306 (BioLegend Cat. No. 849702)

Antigen Details

Structure
Beclin-1 is a 450 amino acid protein with a molecular mass of 52 kD.
Distribution

Tissue distribution: Beclin-1 is ubiquitously expressed. </br>Cellular distribution: Cytosol, endoplasmic reticulum, endosome, mitochondria, and nucleus.

Function
Beclin-1 is involved in autophagosome formation in autophagy.
Interaction
Beclin-1 interacts with Bcl-2 and PI3K class III.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References
  1. Cao Y, et al. 2007. Cell. Research 17:839.
Gene ID
8678 View all products for this Gene ID
UniProt
View information about Beclin-1 on UniProt.org
Go To Top Version: 2    Revision Date: 08.29.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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