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- Clone
- O93F3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ATG6 Autophagy Related 6 Homolog, VSP30
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot of purified anti-Beclin-1 antibody (clone O93F3). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 849701 | 25 µg | 118 CHF | ||||
| 849702 | 100 µg | 288 CHF | ||||
Beclin-1 plays a central role in autophagy and is involved in autophagosome formation. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Beclin-1 is necessary for the initiation of autophagy, and it has been shown to be reduced in the brains of patients with Alzheimer’s disease. Reduced Beclin-1 levels lead to decreased autophagy, the accumulation of Aβ peptides, and neurodegeneration in mouse models of Alzheimer's disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length recombinant human Beclin-1 protein (NP_003757) produced in HEK293T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Deng X, et al. 2017. Biochim. Biophys. Acta. 1862(3):337-350 (WB).
- Product Citations
-
- RRID
-
AB_2687306 (BioLegend Cat. No. 849701)
AB_2687306 (BioLegend Cat. No. 849702)
Antigen Details
- Structure
- Beclin-1 is a 450 amino acid protein with a molecular mass of 52 kD.
- Distribution
-
Tissue distribution: Beclin-1 is ubiquitously expressed. </br>Cellular distribution: Cytosol, endoplasmic reticulum, endosome, mitochondria, and nucleus.
- Function
- Beclin-1 is involved in autophagosome formation in autophagy.
- Interaction
- Beclin-1 interacts with Bcl-2 and PI3K class III.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
- Molecular Family
- Autophagosome Markers
- Antigen References
-
- Cao Y, et al. 2007. Cell. Research 17:839.
- Gene ID
- 8678 View all products for this Gene ID
- UniProt
- View information about Beclin-1 on UniProt.org
Related FAQs
Other Formats
View All Beclin-1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Beclin-1 | O93F3 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Beclin-1
Western blot of purified anti-Beclin-1 antibody (clone O93F3...
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