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Purified anti-β-actin Antibody

Pricing & Availability
Clone
2F1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A_2F1-1_Purified_Beta-Actin_Antibody_1_WB_080316
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β actin antibody (clone 2F1-1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • A_2F1-1_Purified_Beta-Actin_Antibody_1_WB_080316
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β actin antibody (clone 2F1-1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • B_2F1-1_PURE_BetaActin_Ab_IF_022018
    Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml of purified anti- β-actin (clone 2F1-1) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • C_2F1-1_PURE_Beta_Actin_Antibody_052022
    IHC staining using purified anti-β-actin antibody (clone 2F1-1) on formalin-fixed paraffin-embedded human kidney tissue. The tissue was incubated with 5 μg/mL of anti-β-actin antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 μM.
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643801 25 µg 101 CHF
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643802 100 µg 229 CHF
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.
  4. KishidaT,et al. 2007.J. Immunol. 179:8554.
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037.
  9. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  10. Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
  11. Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
Product Citations
  1. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  2. Herr SA, et al. 2022. Neural Regen Res. 17:1505. PubMed
  3. Fathi M, et al. 2023. iScience. 26:106482. PubMed
  4. Tetikoglu S, et al. 2023. Chem Biodivers. 20:e202300401. PubMed
  5. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  6. Kaya-Sezginer E, et al. 2022. Andrology. 10:1441. PubMed
  7. Greenberg D, et al. 2015. Infect Immun . 83: 4277 - 4292. PubMed
  8. Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
  9. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  10. Rodríguez E, et al. 2022. Commun Biol. 5:41. PubMed
  11. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  12. Hadjicharalambous MR, et al. 2019. Sci Rep. 9:6020. PubMed
  13. Kaieda S, et al. 2010. J Biol Chem. 285:21478. PubMed
  14. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  15. Bruggen R, et al. 2015. PLoS One. 10: 0144555. PubMed
  16. Budida R, et al. 2017. Eur J Immunol. 47:1819. PubMed
  17. Scorza M 2011. J Leukoc Biol. 89:159. PubMed
  18. Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed
  19. Lai CC, et al. 2022. Biomed Rep. 16:19. PubMed
  20. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  21. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  22. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  23. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  24. Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
  25. Lalor R, et al. 2019. Nutrients. 11. PubMed
RRID
AB_2223201 (BioLegend Cat. No. 643801)
AB_2223201 (BioLegend Cat. No. 643802)

Antigen Details

Structure
Actin, cytoplasmic 1
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 05.20.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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