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Purified anti-β-Amyloid, 1-38 Antibody (Previously Covance catalog# SIG-39162)

Pricing & Availability
Clone
7-14-4 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# SIG-39162
Isotype
Rabbit Ig
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
808601 50 µg 431 CHF
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Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

ELISA, WB, IHC
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000 - 2,000*
• ELISA: Can be used as capture antibody in a sandwich ELISA with 4G8 detection (Ref Cat. No. 800707 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody, HRP Labeled, or Cat. No. 800701 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody).
For capture antibody; recommended usage is between 1.0 and 10.0 µg/ml. Recommended blocking buffer is 0.5-1.0% BSA in PBS.
If using Cat. No. 800707 for detection, recommended usage is between 0.25 - 1.0 µg/ml.
• IHC: 1:50 - 1:100 with Biotin-based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501)

Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: 70% Formic Acid for 20 minutes at room temperature
Primary Incubation: 24 hours at 2-4°C

Application Notes

This antibody is effective in immunoblotting (WB), ELISA, and immunohistochemistry (IHC).

WB Positive Control tissue: Alzheimer's brain lysate
*Expected MW: Beta Amyloid 38 (~4 kD)
IHC Positive Control tissue: Human Alzheimer's Brain

This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform that ends at the 38th amino acid.

Application References

(PubMed link indicates BioLegend citation)
  1. Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA 28:10323. (IF) PubMed
  2. Giaccone G, et al. 2012. Acta. Neuropathol. 124:809. (IHC, IF, WB)
Product Citations
  1. Watts J, et al. 2014. Proc Natl Acad Sci U S A. 111:10323-10328. PubMed
  2. Condello C, et al. 2018. Proc Natl Acad Sci U S A. 115:E782. PubMed
RRID
AB_2564771 (BioLegend Cat. No. 808601)
Disclaimer

Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-38 on UniProt.org
Go To Top Version: 6    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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