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Purified anti-Bin1 Antibody

Pricing & Availability
Clone
99D (See other available formats)
Regulatory Status
RUO
Other Names
Amphiphysin 2, AMPHL, SH3P9, Box-Dependent Myc-Interacting Protein 1, Myc Box-Dependent-Interacting Protein 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
99D_Purified_Bin1_Antibody_WB_060118
Total lysates (15 µg protein) from U87-MG, HeLa and HL-60 were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-Bin1 antibody, clone 99D. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • 99D_Purified_Bin1_Antibody_WB_060118
    Total lysates (15 µg protein) from U87-MG, HeLa and HL-60 were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-Bin1 antibody, clone 99D. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
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655602 100 µg 265 CHF
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Description

Bridging integrator 1 (Bin1) is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, cell polarity, programmed cell death, and stress signaling. It is involved in membrane remodeling and is one of the important genes in predicting risk for Alzheimer's disease (AD). Alternative splicing of the gene results in ten transcript variants. Isoforms 1-7 are expressed predominantly in the central nervous system. Isoform 8 is expressed specifically in muscle, and defects in BIN1 cause autosomal recessive myotubular myopathy. Isoforms 9 and 10 are ubiquitously expressed and may act as tumor repressors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fragment of human Bin1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, FC, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 4.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Chapuis J, et al. 2013. Mol. Psychiatry doi: 10.1038/mp.2013.1.
RRID
AB_2562162 (BioLegend Cat. No. 655602)

Antigen Details

Structure
Ten isoforms, 409-593 amino acids, predicted molecular weight of 45-70 kD
Distribution

Isoforms 1-7 predominantly cytosolic, isoforms 8-10 found in both the nucleus and/or cytosol

Function
Plays important roles in membrane remodeling, cell polarity, programmed cell death, and stress signaling
Interaction
Heterodimer with AMPH; binds SH3GLB1, SYNJ1, DNM1, clathrin and AP2A2; interacts with MYC, BIN2 and HCV NS5A.
Ligand/Receptor
SH3GLB1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, DNA Repair/Replication, Immunology, Signal Transduction, Transcription Factors
Antigen References

1. Kingwell K. 2013. Nature Reviews Neurology. 9:184.
2. Chapuis J, et al. 2013. Mol. Psychiatry doi: 10.1038/mp.2013.1.
3. Schellenberg GD and Montine TJ. 2012. Acta. Neuropathol. 124:305.
4. Ganesan S. 2011. Sci. Signal. 4:15.
5. Cowling BS, et al. 2012. PLoS. Genet. 8:e1002595.
6. Lambert JC and Amouyel P. 2011. Curr. Opin. Genet. Dev. 21:295.
7. Romero NB. 2010. Neuromuscul. Disord. 20:223.
8. Prendergast GC, et al. 2009. Biochim. Biophys. Acta. 1795:25.

Gene ID
274 View all products for this Gene ID
UniProt
View information about Bin1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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