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Purified anti-BRAP Antibody

Pricing & Availability
Clone
1E7-C9-D10-B10 (See other available formats)
Regulatory Status
RUO
Other Names
BRCA1 Associated Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1E7-C9-D10-B10_PURE_BRAP_Antibody_1_021920
Western blot of purified anti-BRAP antibody (clone 1E7-C9-D10-B10). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1E7-C9-D10-B10_PURE_BRAP_Antibody_1_021920
    Western blot of purified anti-BRAP antibody (clone 1E7-C9-D10-B10). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1E7-C9-D10-B10_PURE_BRAP_Antibody_2_021920
    IHC staining of purified anti-BRAP antibody (clone 1E7-C9-D10-B10) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1E7-C9-D10-B10_PURE_BRAP_Antibody_3_021920
    IHC staining of purified anti-BRAP antibody (clone 1E7-C9-D10-B10) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
871001 25 µg 106 CHF
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871002 100 µg 276 CHF
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Description

BRCA1 associated protein (BRAP) is a cytosolic protein that negatively regulates MAP kinase activation by inhibiting the formation of the Raf/MEK complex. BRAP also inhibits nuclear translocation and promotes the retention of several proteins, including BRCA1, in the cytoplasm by binding to their nuclear localization signal sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human BRAP protein fragments expressed in E. coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2832876 (BioLegend Cat. No. 871001)
AB_2832876 (BioLegend Cat. No. 871002)

Antigen Details

Structure
BRAP is an 592 amino acid protein with predicted and observed molecular mass of ~67 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus, Cytosol

Function
Protein trafficking, Epigenetics
Interaction
BRCA1, Galectin-2
Cell Type
Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Innate Immune Signaling
Antigen References
  1. Modell SM, et al. 2014. J Relig Health. 53(3):715.
  2. Castro E & Eeles R. 2012. Asian J Androl. 14(3):409.
  3. Pristauz G, et al. 2010. Wien Med Wochenschr. 160(7-8):158.
Gene ID
8315 View all products for this Gene ID 72399 View all products for this Gene ID
UniProt
View information about BRAP on UniProt.org

Other Formats

View All BRAP Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-BRAP 1E7-C9-D10-B10 WB,IHC-P
Go To Top Version: 1    Revision Date: 02.19.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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