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Purified anti-BRST-2 (GCDFP-15) Antibody

Pricing & Availability
Clone
D6 (See other available formats)
Regulatory Status
RUO
Other Names
Prolactin-inducible protein (PIP), secretory actin-binding protein, gross cystic disease fluid protein 15, BRST2, GCDFP15, GCDFP-15, GPIP4
Isotype
Mouse IgG2a, κ
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Product Citations
publications
D6_PURE_BRST-2_Antibody_IHC-P_022819
IHC staining of anti-BRST-2 (GCDFP-15) antibody (clone D6) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
  • D6_PURE_BRST-2_Antibody_IHC-P_022819
    IHC staining of anti-BRST-2 (GCDFP-15) antibody (clone D6) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
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915106 100 µg 229 CHF
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Description

The BRST-2 antigen is expressed by apocrine sweat glands, eccrine glands (variable), minor salivary glands, bronchial glands, metaplasmic epithelium of the breast, benign sweat gland tumors of the skin and the serous cells of the submandibular gland. Breast carcinomas (primary and metastatic lesions) with apocrine features express the BRST-2 antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against Gross Cystic Disease Fluid Protein 15.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The BRST-2 clone D6 recognizes a 15 kD glycoprotein known as Gross Cystic Disease Fluid Protein-15 (GCDFP-15). Antigen retrieval is not required. The BRST-2 epitope is heat-sensitive; paraffin sections that have been heat fixed at 60°C may lose antibody binding sites.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshimura Y, et al. 2011. Jpn. J. Clin. Oncol. 41:358. (IHC) PubMed
  2. Fan CY, et al. 2000. Am. J. Surg. Pathol. 24(4):579. PubMed
  3. Wick MR, et al. 1989. Hum. Pathol. 20(3):281. PubMed
  4. Mazoujian G, et al. 1984. Am. J. Surg. Pathol. 8(1):43. PubMed
  5. Mazoujian G, et al. 1983. Am. J. Pathol. 110:105. PubMed
  6. Brown RW, et al. 1997. Am. J. Clin. Path. 107:12. PubMed
RRID
AB_2616964 (BioLegend Cat. No. 915106)

Antigen Details

Structure
BRST-2 (GCDFP-15) is a 17 kD protein.
Distribution

Extracellular and nucleus.

Function
Diseases associated with BRST-2 include florid papillomatosis of the nipple and breast signet ring cell adenocarcinoma. Interacts with AZGP1.
Biology Area
Cancer Biomarkers, Cell Biology
Gene ID
5304 View all products for this Gene ID
UniProt
View information about BRST-2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06.10.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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