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Purified anti-c-Myb Antibody

Pricing & Availability
Clone
W16209A (See other available formats)
Regulatory Status
RUO
Other Names
Myb, c-Myb, Transcriptional Activator Myb, Proto-oncogene c-Myb, MYB Proto-Oncogene, Transcription Factor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16209A_PURE_c-Myb_Antibody_1_022420
Whole cell extracts (15 µg protein) from the indicated cell lines, A549 (negative control), RPMI 8226, U-937, THP-1, Jurkat, and MOLT-4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-c-Myb antibody (clone W16209A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker
  • W16209A_PURE_c-Myb_Antibody_1_022420
    Whole cell extracts (15 µg protein) from the indicated cell lines, A549 (negative control), RPMI 8226, U-937, THP-1, Jurkat, and MOLT-4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-c-Myb antibody (clone W16209A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker
  • W16209A_PURE_c-Myb_Antibody_2_022420
    Whole cell extracts (300 μg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 μg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400501) or purified anti-c-Myb antibody (clone W16209A). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of W16209A. Lane M: Molecular weight marker.
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935301 25 µg 101 CHF
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935302 100 µg 253 CHF
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Description

c-Myb is a proto-oncogene protein that functions as a transcription factor and a key regulator of cell proliferation and differentiation. As a DNA binding protein, it plays a role in regulating cell cycle and gene expression. c-Myb expression is elevated in hematopoietic progenitor cells and decreases following cell differentiation. Increased or aberrant expression of c-Myb as well as duplication and c-terminal deletion have been reported in various malignancies suggesting it plays a critical role in tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein of human MYB corresponding to amino acid residues 391-640
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  • This clone is predicted to recognize isoforms 1, 2, 4, 7, and 8 based off complete sequence homology of the immunizing peptide and the corresponding region in each isoform.
  • ICC test failed. The clone incorrectly stains the negative control in the cytosol. 
RRID
AB_2832893 (BioLegend Cat. No. 935301)
AB_2832893 (BioLegend Cat. No. 935302)

Antigen Details

Structure
Myb is a 640 amino acid protein with a predicted molecular weight of 72 kD.
Distribution

Nucleus/differential expression 

Function
Gene transcription, cell proliferation and differentiation
Cell Type
Epithelial cells, Leukemia
Biology Area
Cell Biology, Stem Cells, Transcription Factors
Antigen References
  1. Schulz C, et al. 2012. Science. 336(6077):86-90.
  2. Martin C, et al. 1997. Trends Genet. 13(2):67-73.
  3. Mucenski M, et al. 1991. Cell. 65(4):677-89.
Gene ID
4602 View all products for this Gene ID
UniProt
View information about c-Myb on UniProt.org
Go To Top Version: 1    Revision Date: 02.13.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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