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Purified anti-c-REL Antibody

Pricing & Availability
Clone
2F5A22 (See other available formats)
Regulatory Status
RUO
Other Names
REL, V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog, Proto-Oncogene C-Rel
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_2F5A22_Purified_c-Rel_Antibody_Updated_103017
Total lysates (15 µg protein) from HeLa (lane 1), Daudi (lane 2), and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-c-REL Antibody, clone 2F5A22 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-REL Antibody. 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody (2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • 1_2F5A22_Purified_c-Rel_Antibody_Updated_103017
    Total lysates (15 µg protein) from HeLa (lane 1), Daudi (lane 2), and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-c-REL Antibody, clone 2F5A22 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-REL Antibody. 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody (2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • 2_2F5A22_GoChIP_c-REL_Antibody_042617.jpg
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells treated with TPA and Ionomycin with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-c-REL (Clone 2F5A22), B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-c-REL Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Bcl2L1 gene region and human αSatellite repeat (as a negative control). The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
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655802 100 µg 347 CHF
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Description

c-Rel is a member of the Rel/NF-κB transcription factor family that forms homo or heterodimers with family members such as RelA/p65, RelB, NF-κB1/p105, and NF-κB2/p100. The NF-κB complex is retained in cytoplasm by the inhibitor IκB protein in unstimulated cells. Upon activation, IκB is degraded via the ubiquitin-proteasome pathway, which results in nuclear translocation of the active NF-κB complex. While other NF-κB transcription factors are expressed in most cell types, relatively higher levels of c-REL expression are found in hematopoietic cells. c-REL is thought to play key roles in regulating proliferation, differentiation, and survival of B and T lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial c-REL recombinant protein (453-587 a.a.)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562203 (BioLegend Cat. No. 655802)

Antigen Details

Structure
619 amino acids, predicted molecular weight of 69 kD, contains a RHD (Rel-like) domain responsible for dimerization and DNA binding, contains two C-terminal transactivation domains
Distribution

Cytoplasm, translocated to the nucleus upon activation

Function
Transcription factor belonging to the NF-κB family, regulates various biological processes including tumorgenesis, inflamation, immune response, cell proliferation, and differentiation
Interaction
Forms homo or heterodimer with other NF-κB family members; interacts with NKIRAS1, NFKBIB, NFKBIE, IRF-8, and Foxp3
Cell Type
B cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers, TCRs
Antigen References

1. Bash J, et al. 1997. Mol. Cell Biol. 17:6526.
2. Chen G, et al. 2010. J. Immunol. 185:2350. 
3. Gilmore TD, et al. 2011. Genes Cancer 2:695. 
4. Ferch U, et al. 2007. Nat. Immunol. 8:984. 
5. Sanjabi S, et al. 2005. Genes Dev. 19:2138.
6. Yamazaki T, et al. 2003. Nat. Immunol. 4:780.

Gene ID
5966 View all products for this Gene ID
UniProt
View information about c-REL on UniProt.org
Go To Top Version: 4    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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