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Purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP) Antibody

Pricing & Availability
Clone
7F8C76 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7F8C76_PURE_C2H2zinc_Antibody_1_WB_071519
Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 1.0 µg/mL (1:500 dilution) of purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP), clone 7F8C76, 2 hrs at RT. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • 7F8C76_PURE_C2H2zinc_Antibody_1_WB_071519
    Whole cell extracts (15 µg protein) from serum starved HeLa untreated (-) or treated (+) with 200 ng/mL Nocodazole for 20 hrs were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. The membrane was treated (+ LPP) or untreated (- LPP) with lambda protein phosphatase, overnight at 4°C and probed with 1.0 µg/mL (1:500 dilution) of purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP), clone 7F8C76, 2 hrs at RT. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • 7F8C76_PURE_C2H2zinc_Antibody_2_IF_072315
    Asynchronized HeLa cells were stained with purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and DAPI (blue). The arrow heads indicate mitotic HeLa cells.
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678902 100 µg 335 CHF
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Description

C2H2 zinc finger proteins (ZFPs) are DNA-binding transcription factors that regulate a variety of biological processes such as cellular growth, differentiation, and proliferation. A zinc finger protein can contain several tandems of clustered zinc finger motifs responsible for DNA binding. However, stable ZFP binding to DNA requires the conserved linker sequences that join adjacent zinc fingers. These linkers participate in wrapping of the ZFP around the double helix of DNA, which is required for efficient binding. Most of the ZFP linkers contain the same amino acid sequence HTGEKP, which has been shown to be phosphorylated at the threonine residue by TOPK/PBK during mitosis. Phosphorylation on the linker sequence of ZFP leads to the inactivation of DNA binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide (HQRIH(pT)GEKPYKC) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566052 (BioLegend Cat. No. 678902)

Antigen Details

Structure
Conserved linker region (HTGEKP) of C2H2 zinc finger proteins.
Distribution

Mitotic cells.

Function
The linker region (HTGEKP) of C2H2 zinc finger proteins facilitate stable DNA binding and is highly conserved among these zinc finger proteins. During mitosis, C2H2 zinc finger proteins are phosphorylated on the threonine residue of the linker peptide, which result in the inactivation of their DNA binding activity.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Dovat S, et al. 2002. Genes Dev. 16:2985.
2. Rizkallah R, et al. 2011. Cell cycle 10:3327.
3. Rizkallah R, et al. 2015. Oncotarget 6:1446.

Gene ID
NA
UniProt
View information about C2H2 zinc finger proteins on UniProt.org
Go To Top Version: 3    Revision Date: 07.15.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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