Loading...

Purified anti-Calponin 1 Antibody

Pricing & Availability
Clone
CALP (See other available formats)
Regulatory Status
RUO
Other Names
Calponin 1, basic, smooth muscle (CNN1), Epididymis Secretory Protein Li 14, SMCC, Sm-Calp, HEL-S-14, Basic calponin, Calponin H1 smooth muscle
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
a. CALP_PURE_Calponin-1_WB_IHC-P_021125
IHC staining with Purified anti-Calponin 1 (clone CALP) on formalin-fixed paraffin-embedded human uterus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) Purified anti-Calponin 1 (clone CALP) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • a. CALP_PURE_Calponin-1_WB_IHC-P_021125
    IHC staining with Purified anti-Calponin 1 (clone CALP) on formalin-fixed paraffin-embedded human uterus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) Purified anti-Calponin 1 (clone CALP) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • B. CALP_PURE_Calponin-1_ICC_021125
    A-431 cells (low expression cell line, panels A and C) and Saos-2 cells (high expression cell line, panels B and D) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Calponin 1 (clone CALP), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (Panels A and C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. CALP_PURE_Calponin-1_WB_021125
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Calponin 1 (clone CALP) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • D. CALP_PURE_Calponin-1_WB_ICFC_021125
    U-2 OS cells (positive cell line, filled histogram) and HeLa cells (low-expressing cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Purified anti-Calponin 1 (clone CALP), or Purified Mouse IgG1, κ Isotype Control (Cat. No. 401402) (open histogram, dashed line is representative for both U-2 OS and HeLa cells).
Cat # Size Price Quantity Check Availability Save
692051 25 µg 180 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
692052 100 µg 450 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Calponin 1, encoded by the CNN1 gene, is an actin filament-associated regulatory protein found primarily in smooth muscle but also in non-muscle cells. Calponin 1 plays a significant role in regulating the contractility and structural integrity of the cytoskeleton by binding to actin and actin-interacting proteins, modulating their interplay. For example, by binding to actin and inhibiting myosin light chain kinase Calponin 1 reduces the phosphorylation of myosin light chains, preventing excessive contraction and ensuring appropriate smooth muscle response to physiological stimuli. Furthermore, Calponin 1 has been implicated in various physiological functions beyond muscle contraction including vascular tone and wound healing, making it a key player in maintaining homeostasis in various tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Crude human uterus extract
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:

- Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
- Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
- 100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization with either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.

This antibody does not cross react with mouse Calponin 1 in Western Blot.

RRID
AB_3683499 (BioLegend Cat. No. 692051)
AB_3683499 (BioLegend Cat. No. 692052)

Antigen Details

Structure
Calponin 1 is a 297 amino acid protein with a calculated molecular weight of 33.1 kD.
Distribution

Cytoplasmic, cytoskeleton

Interaction
Actin, tropomyosin, myosin, calmodulin
Biology Area
Cardiovascular Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family
Microfilaments
Antigen References
  1. Wu K and Jin J. 2008. Cell Biochem Biophys. 52:139-48.
  2. Liu R and Jin JP. 2016. Gene. 585:143-153.
  3. Winder SJ and Walsh M. 1993. Cell Signal. 5:677-86.
Gene ID
1264 View all products for this Gene ID
UniProt
View information about Calponin 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.11.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account