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Purified anti-CAMK IV Antibody

Pricing & Availability
Clone
P88C5B8 (See other available formats)
Regulatory Status
RUO
Other Names
Calcium/calmodulin-dependent protein kinase type IV, CaM kinase-GR, CAMK4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P88C5B8_PURE_CAMKIV_Antibody_WB_09142016
Total cell or tissue lysates (15 µg protein) from EL-4, Jurkat, HepG2, and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-CAMK IV antibody (clone P88C5B8) antibody (upper) or loading control GAPDH (clone poly6314) antibody (lower). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • P88C5B8_PURE_CAMKIV_Antibody_WB_09142016
    Total cell or tissue lysates (15 µg protein) from EL-4, Jurkat, HepG2, and NIH3T3 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-CAMK IV antibody (clone P88C5B8) antibody (upper) or loading control GAPDH (clone poly6314) antibody (lower). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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691102 100 µg 253 CHF
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Description

Calcium/calmodulin-dependent protein kinase IV (CaMK IV) is a multifunctional serine/threonine kinase regulating different cellular processes. CaMK IV resides in both the nucleus and cytosol, but the active phosphorylated form of CaMK IV is predominantly nuclear. Once activated, CAMK IV enhances BCL2 gene expression. CaMK IV also actively regulates gene transcription by phosphorylating HDAC4. Phosphorylation of HDAC4 by CaMK IV promotes HDAC4 nuclear exports in neurons, resulting in major cell death mechanism in cerebral ischemia. CaMK IV–mTOR-dependent autophagy was fundamentally important for IL-6 production in vitro and in vivo.

CaMK IV is abnormally increased in T cells from patients with SLE and lupus-prone mice. Genetic or pharmacologic inhibition of CaMK IV resulted in a significant decrease of autoantibody production, mesangial cell proliferation, improved Treg function, improvement of lupus-related pathology, and the survival rates. CaMK IV inhibition has potential as a therapeutic strategy for Th17-driven autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human CAMK4 (AA:35-292) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629828 (BioLegend Cat. No. 691102)

Antigen Details

Structure
473 amino acids with an expected molecular weight of 52 kD.
Distribution
Cytoplasm and the nucleus. Expressed in the brain, thymus, CD4 T-cells, testis, and epithelial ovarian cancer tissue.
Function
In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG, and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2.
Interaction
Phosphatase 2A (PPP2CA/PPP2CB)
Antigen References

1. Racioppi L, et al.  2008. Trends Immunol 29:600.
2. Zhang, et al. 2014. J. Immunol. 193:2405.
3. McCullough LD, et al. 2013. Stroke. 44:2559.
4. Bolger TA, et al. 2005. J. Neurosci. 25:9544.
5. Juang YT, et al. 2005. J. Clin. Invest. 115:996.
6. Koga T, et al. 2014. J. Clin. Invest. 124:2234.

Gene ID
814 View all products for this Gene ID
UniProt
View information about CAMK IV on UniProt.org
Go To Top Version: 1    Revision Date: 09.16.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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