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Purified anti-Caspase-1 Antibody

Pricing & Availability
Clone
5B10 (See other available formats)
Regulatory Status
RUO
Other Names
ICE
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5B10_Purified_Caspase-1_Antibody_WB_101716
Total cell lysates (15 µg protein) from Jurkat and P338D1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Caspase 1 antibody (clone 5B10). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. GAPDH Antibody was used as a loading control.
  • 5B10_Purified_Caspase-1_Antibody_WB_101716
    Total cell lysates (15 µg protein) from Jurkat and P338D1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Caspase 1 antibody (clone 5B10). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. GAPDH Antibody was used as a loading control.
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645101 25 µg 124 CHF
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645102 100 µg 265 CHF
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Description

Caspase -1 plays a vital role in cleaving the inactive procytokine pro-IL-1 beta to generate the active 17 kd mature form of IL-1beta, a key mediator of inflammation. Active caspase-1 (ICE) is derived a single proenzyme of 45 Kd. An active ICE comprises two subunits of P20 and two subunits of P10 after removal a large amino-terminal pro-domain and a small linker peptide. Caspase-1 is reported to be involved in CD95- mediated apoptosis. Four alternatively spliced variants of caspase-1 have been reported, with mw ranging from 30-48 kd.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Bacterial fusion protein (p20)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1-2µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang SJ, et al. 2000. J Cell Biol 149:613
  2. Kang SJ, et al. 2002. Cell Death and Differentiation. 9:1115
Product Citations
  1. Zhao YJ, et al. 2022. Neural Regen Res. 17:1324. PubMed
  2. Liu L, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjw219. PubMed
  3. Zhu Q, et al. 2016. Am J Physiol Renal Physiol. 310: F621-F627. PubMed
  4. Reis AS, et al. 2020. Sci Adv. 6:eaax6346. PubMed
  5. Nastase MV, et al. 2016. The American Journal of Pathology. 186(5):1206-20. PubMed
RRID
AB_2068900 (BioLegend Cat. No. 645101)
AB_2068900 (BioLegend Cat. No. 645102)

Antigen Details

Structure
The whole length of Caspase-1 is a 404 amino acid polypeptide chain with MW of 45kd. The active Caspase -1 is a tetramer compariseing two subunits of 20Kd and 10Kd.
Distribution

Predominantly in the cytoplasm, some localized to the external cell membrane.

Function
Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta.
Ligand/Receptor
Pro-IL1 beta
Bioactivity
IL-1beta converting enzyme (ICE)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Wilson KP et al. 1994. Nature 370:251
2. Cohen GM:1997 Biochem J 326:1

Gene ID
834 View all products for this Gene ID
UniProt
View information about Caspase-1 on UniProt.org
Go To Top Version: 4    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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