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Purified anti-Caspase-11 Antibody

Pricing & Availability
Clone
Cas11.17D9 (See other available formats)
Regulatory Status
RUO
Other Names
Casp11
Isotype
Rat IgG2a
Ave. Rating
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Product Citations
publications
cas11.17D9_caspase-11_Purified_Antibody_WB_012114.jpg
Western blot analysis of Raw264.7 cells starved overnight(lane 1), Raw264.7 cells starved overnight and treated with 1 µg/ml LPS for nine hours (lane 2) and NIH3T3 untreated cells (lane 3) using anti-Caspase11 antibody (clone cas11.17D9).
  • cas11.17D9_caspase-11_Purified_Antibody_WB_012114.jpg
    Western blot analysis of Raw264.7 cells starved overnight(lane 1), Raw264.7 cells starved overnight and treated with 1 µg/ml LPS for nine hours (lane 2) and NIH3T3 untreated cells (lane 3) using anti-Caspase11 antibody (clone cas11.17D9).
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647201 25 µg 118 CHF
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647202 100 µg 293 CHF
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Description

Murine caspase-11 is an upstream protease of caspase-1 and interacts with the proform of caspase-1 which in turn activates IL-1 beta. Caspase-11 is not constitutively expressed in most cell types. Its expression can be upregulated by LPS, IFN-gamma, and other pro-inflammatory stimuli. NF-κB and STAT1 were reported to be involved in the activation. Caspase-11 knock -out mice were resistant to endotoxin shock and do not produce IL-1 after stimulation with LPS. Their production of IFN-gamma was also deficient.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
His tagged bacterial fusion protein of p 30 of caspase 11
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): 1 to 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schmidt RL, et al. 2012. PLoS One. 7:e45186. PubMed.
Product Citations
  1. Khameneh H, et al. 2011. PLoS One. 6:e23025. PubMed
  2. Schmidt R, Lenz L 2012. PLoS One. 7:e45186. PubMed
  3. Min L, et al. 2012. J Immunol. 188:1789. PubMed
  4. Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
RRID
AB_1937283 (BioLegend Cat. No. 647201)
AB_1937283 (BioLegend Cat. No. 647202)

Antigen Details

Structure
This murine caspase 11 has pro-enzymes a 38 and 43 kD. The activated form consists of p30 and p10.
Distribution
Not constitutively expressed in most cell types, but can be induced by different pro-inflmmatory stimuli including LPS.
Function
Essential for the activation of caspase-1, thus involved in the maturation of IL-1beta and IL-18. Both are important mediators of inflammation.
Interaction
Caspase-1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Lee J et al. 2001. J. Biol. Chem. 276:32956
2. Schauvliege R et al. 2002. J. Biol. Chem. 277:41624
3. Wang S et al. 1998. Cell 92:501

Gene ID
201161 View all products for this Gene ID
UniProt
View information about Caspase-11 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 01.21.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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