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- Clone
- Cas11.17D9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Casp11
- Isotype
- Rat IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot analysis of Raw264.7 cells starved overnight(lane 1), Raw264.7 cells starved overnight and treated with 1 µg/ml LPS for nine hours (lane 2) and NIH3T3 untreated cells (lane 3) using anti-Caspase11 antibody (clone cas11.17D9).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 647201 | 25 µg | 118 CHF | ||||
| 647202 | 100 µg | 293 CHF | ||||
Murine caspase-11 is an upstream protease of caspase-1 and interacts with the proform of caspase-1 which in turn activates IL-1 beta. Caspase-11 is not constitutively expressed in most cell types. Its expression can be upregulated by LPS, IFN-gamma, and other pro-inflammatory stimuli. NF-κB and STAT1 were reported to be involved in the activation. Caspase-11 knock -out mice were resistant to endotoxin shock and do not produce IL-1 after stimulation with LPS. Their production of IFN-gamma was also deficient.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- His tagged bacterial fusion protein of p 30 of caspase 11
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): 1 to 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Schmidt RL, et al. 2012. PLoS One. 7:e45186. PubMed.
- Product Citations
-
- RRID
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AB_1937283 (BioLegend Cat. No. 647201)
AB_1937283 (BioLegend Cat. No. 647202)
Antigen Details
- Structure
- This murine caspase 11 has pro-enzymes a 38 and 43 kD. The activated form consists of p30 and p10.
- Distribution
- Not constitutively expressed in most cell types, but can be induced by different pro-inflmmatory stimuli including LPS.
- Function
- Essential for the activation of caspase-1, thus involved in the maturation of IL-1beta and IL-18. Both are important mediators of inflammation.
- Interaction
- Caspase-1
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
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1. Lee J et al. 2001. J. Biol. Chem. 276:32956
2. Schauvliege R et al. 2002. J. Biol. Chem. 277:41624
3. Wang S et al. 1998. Cell 92:501 - Gene ID
- 201161 View all products for this Gene ID
- UniProt
- View information about Caspase-11 on UniProt.org
Other Formats
View All Caspase-11 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Caspase-11 | Cas11.17D9 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Caspase-11
Western blot analysis of Raw264.7 cells starved overnight(la...
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