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Purified anti-CCL24 (Eotaxin-2) Antibody

Pricing & Availability
Clone
A15037C (See other available formats)
Regulatory Status
RUO
Other Names
Eotaxin2, MPIF2, MPIF-2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15037C_PURE_CCL24_Antibody_WB_022216
Recombinant Human CCL24 (Eotaxin-2, carrier-free) (50ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-CCL 24 (clone A15037C) antibody. Protein was visualized using a HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • A15037C_PURE_CCL24_Antibody_WB_022216
    Recombinant Human CCL24 (Eotaxin-2, carrier-free) (50ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-CCL 24 (clone A15037C) antibody. Protein was visualized using a HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
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683602 100 µg 265 CHF
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Description

CLL24 was initially cloned from a library of activated macrophages. Using human eosinophils, it was shown that it binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). In addition, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7. Eotaxin/CCR3 expression has been studied extensively in the pathogenesis of asthma and allergies. The eotaxin/CCR3 axis has been associated to inflammatory and autoimmune disorders including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis. CCL24 has also been associated to neovascular age-related macular degeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CCL24
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572025 (BioLegend Cat. No. 683602)

Antigen Details

Structure
93 amino acids with a predicted molecular weight of 10.4 KD.
Distribution
CCL24 is expressed by epithelial cells, airway smooth muscle cells, and vascular endothelial cells. In addition, eotaxins are produced by cells isolated by bronchial alveolar lavage, including macrophages, monocytes, basophils, eosinophils, and lymphocytes.
Function
CCL24 attracts CCR3+ cells such as eosinophils, mast cells, basophils, and Th2 cells that play a role in allergic disorders. IL-4 and IL-13 induce CCL11 and CCL24 in esophagus and colonic epithelial cell, and IL-13 induces the production of CCL24 in dendritic cells and alveolar macrophages. In addition, CCL24 is induced by IL-33 in lung and by IL-4 and LPS in monocytes and macrophages. The expression of CCL24 by alveolar epithelial cells requires histamine and its receptor H2R.
Interaction
Eosinophils, mast cells, Th2 lymphocytes, basophils, human dendritic cells, synovial tissue macrophages, lung epithelial cells, and airway smooth muscle cells.
Ligand/Receptor
CCR3
Cell Type
Epithelial cells, Endothelial cells, Macrophages, Monocytes, Basophils, Eosinophils, Lymphocytes
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Patel VP, et al. 1997. J. Exp. Med. 185:1163.
2. Beaulieu S, et al. 2002. J. Immunol. 169:2925.
3. Watanabe K, et al. 2002. J. Immunol. 168:1911.
4. Ablin JN, et al. 2010. Clin. Exp. Immunol. 161:276.
5. Manousou P, et al. 2010. Clin. Exp. Immunol. 162:337.
6. Stolarski B, et al. 2010. J. Immunol. 185:3472.
7. Crapster-Pregont M, et al. 2012. J. Allergy Clin. Immunol. 129:1621.
8. Swartzendruber JA, et al. 2012. J. Immunol. 188:536.

Gene ID
6369 View all products for this Gene ID
UniProt
View information about CCL24 on UniProt.org
Go To Top Version: 1    Revision Date: 02.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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