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Purified anti-CD105 (Endoglin) Antibody

Pricing & Availability
Clone
O99E5 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Mouse IgG1
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Product Citations
publications
99E5_PURE_CD105_Endoglin_Antibody_WB_080316
Total cell lysate from HUVEC cells (lane 1, 15 µg) and THP-1 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-CD105 (Endoglin) antibody (clone O99E5). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 99E5_PURE_CD105_Endoglin_Antibody_WB_080316
    Total cell lysate from HUVEC cells (lane 1, 15 µg) and THP-1 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-CD105 (Endoglin) antibody (clone O99E5). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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684102 100 µg 124 CHF
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Description

CD105 (Endoglin) is expressed by endothelial cells, pro-fibrogenic cells and hepatic stellate cells; it plays important roles in endothelial proliferation and cardiovascular development. Mutations in the ENG gene lead to Hereditary Hemorrhagic Telangiectasia, a disorder characterized by arteriovenous malformations. An extracellular domain of the full-length membrane endoglin, soluble endoglin, is a proposed biomarker of cardiovascular disease progression, cardiovascular disease treatment and endothelial dysfunction. Endoglin is also expressed in the syncytiotrophoblast throughout pregnancy, but transiently upregulated in the extravillous trophoblast of anchoring villi. Furthermore, endoglin is overexpressed in the tumor-associated vascular endothelium, where it modulates angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein of human Endoglin/CD105 expressed in HEK293T.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Does not react with mouse.

RRID
AB_2572089 (BioLegend Cat. No. 684102)

Antigen Details

Structure
658 amino acid with the predicted molecular weight of approximately 71kD.
Distribution

Endothelial cells, mesangial cells, cardiac and scleroderma fibroblasts, and hepatic stellate cells.

Function
Endoglin plays important roles in endothelial proliferation and cardiovascular development.
Interaction
TGFBR1, TGFBR2, TGF-beta 1, TCTEX1D4, ARRB2 and GDF2.
Ligand/Receptor
TGF-β1, TGF-β3.
Cell Type
Endothelial cells, Fibroblasts
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Rathouska J, et al. 2015. Atherosclerosis 243:383.
2. Kapur NK, et al. 2013. Vasc. Health Risk Manag. 9:195.
3. Meurer SK, et al. 2014. World J. Biol. Chem. 5:180.
4. Gregory AL, et al. 2014. Placenta 35:S93-9.
5. Nassiri F, et al. 2011. Anticancer Res. 31:2283.
5. Zhang Y, et al. 2011. Int. J. Clin. Exp. Med. 4:32-42.

Gene ID
2022 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org
Go To Top Version: 2    Revision Date: 08.03.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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