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Purified anti-CD106 (VCAM-1) Antibody

Pricing & Availability
Clone
A16047A (See other available formats)
Regulatory Status
RUO
Other Names
INCAM-110, VCAM1, VCAM 1, Vascular cell adhesion protein 1, V-CAM 1,
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16047A_PURE_CD106_VCAM-1_Antibody_062017
Total cell lysates (15µg protein) from HUVEC and HUVEC treated with recombinant Human TNF-α (10 ng/ml, 16 h) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:25000 (0.02µg/ml), 1:50000 (0.01µg/ml) and 1:250000 (0.002µg/ml) purified anti-CD106 (VCAM-1) antibody (clone A16047A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A16047A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder, M* indicates longer exposure.
  • A16047A_PURE_CD106_VCAM-1_Antibody_062017
    Total cell lysates (15µg protein) from HUVEC and HUVEC treated with recombinant Human TNF-α (10 ng/ml, 16 h) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:25000 (0.02µg/ml), 1:50000 (0.01µg/ml) and 1:250000 (0.002µg/ml) purified anti-CD106 (VCAM-1) antibody (clone A16047A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for clone A16047A, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder, M* indicates longer exposure.
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698201 25 µg 101 CHF
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698202 100 µg 253 CHF
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Description

CD106 is a glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant VCAM-1/FC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.002 - 0.1 µg/mL (1:5000-1:250000 diluted). It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Flaskamp L, et al. 2022. Cells. 11: . PubMed
  2. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2687276 (BioLegend Cat. No. 698201)
AB_2687276 (BioLegend Cat. No. 698202)

Antigen Details

Structure
Ig superfamily, 81 kD. Six N-glycosylation sites, apparent Mw is 90-110kD.
Distribution

Plasma membrane of bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Important in cell-cell recognition. Function in leukocyte-endothelial cell adhesion.
Interaction
Leukocytes
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leuko. Biol. 57:168.
3. Bevilacqua MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.

Gene ID
7412 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org
Go To Top Version: 0    Revision Date: 06.20.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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