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Purified anti-CD243 (ABCB1, P-glycoprotein 1) Antibody (Previously Covance catalog# SIG-38720)

Pricing & Availability
Clone
C494 (See other available formats)
Regulatory Status
RUO
Other Names
P-glycoprotein 1, ABC20, CLCS, GP170, MDR1, P-GP, PGY1, colchicin sensitivity, doxorubicin resistance, multidrug resistance protein 1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, MRP1, Multidrug resistance protein 1, P-glycoprotein 1
Previously
Signet Catalog# 8720-01
Covance Catalog# SIG-38720
Isotype
Mouse IgG2a, κ
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Product Citations
publications
C494_Purified_CD243_Antibody_IHC_021715
Immunohistochemical staining of CD243 (ABCB1, P-glycoprotein 1), on formalin fixed paraffin embedded normal human liver tissue.
  • C494_Purified_CD243_Antibody_IHC_021715
    Immunohistochemical staining of CD243 (ABCB1, P-glycoprotein 1), on formalin fixed paraffin embedded normal human liver tissue.
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907001 1 mL 604 CHF
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Description

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P, ICC, IP
FC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:20 for Biotin based detection systems such as USA Ultrastreptavidin Detection (Cat. No. 929501)

Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: Not Required.
Incuabation: 20-60 minutes at room temperature.
 

Application Notes

This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunocytochemistry (ICC) and immunopurification (IP).

Positive Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives.
Positive Tissues: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretaory epithelium).

C494 detects a gene-specific, internal cellular epitope (PNTLEGN) present only on the Mdr1 isoform of P-glycoprotein. C494 does not cross react with Mdr3. C494 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant 130kDa mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections. Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein expression. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.

Application References

(PubMed link indicates BioLegend citation)
  1. Georges E, et al.1990. PNAS. 87:152. (IHC, ELISA)
  2. Toth K, et al.1992. Am. J. Pathol. 140:1009. (IHC,ICC,WB,ELISA)
  3. Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed
  4. Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed
  5. Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC, ICC, FC) PubMed
  6. Villar VH, et al. 2012. PLos One. 7:37735. (IHC) PubMed
  7. Mahadevan D, et al. 2013. Cancer. 119:371. (IHC) PubMed
  8. Martin-Broto J, et al. 2014. Mol Cancer Ther. 13:249. (IHC) PubMed
Product Citations
  1. Lee J, et al. 1996. Cancer. 77:1892-1898. PubMed
  2. Mahadevan D, et al. 2013. Cancer. 119:371-379. PubMed
  3. Martin-Broto J, et al. 2014. Mol Cancer Ther. 13:249-259. PubMed
  4. Beck W, et al. 1996. Cancer Res. 56:3010-3020. PubMed
  5. Villar V, et al. 2012. PLoS One. 7:e37735. PubMed
RRID
AB_2565071 (BioLegend Cat. No. 907001)

Antigen Details

Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Gene ID
5243 View all products for this Gene ID
UniProt
View information about CD243 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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