- Clone
- A20079E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cluster of differentiation 276 (CD276), B7 Homolog 3 (B7-H3), 4Ig-B7-H3, B7RP-2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
616951 | 25 µg | 180 CHF | ||||
616952 | 100 µg | 450 CHF |
CD276, or B7-H3, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human CD276 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain and a signature intracellular domain. There also exists an additional isoform known as 4Ig-B7-H3, containing a nearly exact tandem duplication of the IgV-IgC domain. Expression of CD276 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages, and is upregulated during the maturation of monocytes to DCs, or following interactions between DCs and regulatory T cells. CD276 inhibits T-cell responses, and plays a role in adaptive immunity by suppressing T cell activation. CD276 is also expressed in various cancers, where it works to suppress tumor antigen-specific immune responses.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of human CD276
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
FC, ICC, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone A20079E does not cross-react with mouse CD276 in WB.
- RRID
-
AB_3068218 (BioLegend Cat. No. 616951)
AB_3068218 (BioLegend Cat. No. 616952)
Antigen Details
- Structure
- CD276 is a 534 amino acid protein with a predicted molecular weight of 58.2 kD.
- Distribution
-
Dendritic cells, macrophages, natural killer cells, induced T cells. In the tonsil, it is expressed in epithelial cells.
- Function
- Co-stimulatory molecule that inhibits T-cell responses
- Ligand/Receptor
- CD276 binds to Trem-like transcript 2 (TLT-2).
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Inhibitory Molecules, Innate Immunity
- Antigen References
-
- Nagashima O, et al. 2008. J Immunol. 181:4062-71.
- Prasad DVR, et al. 2004. J Immunol. 173:2500-6.
- Sun M, et al. 2002. J Immunol. 168:6294-7.
- Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235-40.
- Ford JW, et al. 2009. Curr Opin Immunol. 21:38-46.
- Leitner J, et al. 2009. Eur J Immunol. 2009. 39:1754-64.
- Gene ID
- 80381 View all products for this Gene ID
- UniProt
- View information about CD276 on UniProt.org
Related FAQs
Other Formats
View All CD276 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CD276 (B7-H3) | A20079E | IHC-P,FC,ICC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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