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Purified anti-CD304 (Neuropilin 1) Antibody

Pricing & Availability
Clone
A18190A (See other available formats)
Regulatory Status
RUO
Other Names
Vascular Endothelial Cell Growth Factor 165 Receptor, Neuropilin-1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A18190A_PURE_CD304_Antibody_050620
Western blot of purified anti-CD304 (Neuropilin 1) antibody (clone A18190A). Lane 1, 5: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 10 µg of MCF7 cell lysate; Lane 4: 10 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. MCF7 and SH-SY5Y cell lysates were loaded in order of decreasing CD304 expression; predicted expression data was obtained from Human Protein Atlas.
  • A18190A_PURE_CD304_Antibody_050620
    Western blot of purified anti-CD304 (Neuropilin 1) antibody (clone A18190A). Lane 1, 5: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 10 µg of MCF7 cell lysate; Lane 4: 10 µg of SH-SY5Y cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. MCF7 and SH-SY5Y cell lysates were loaded in order of decreasing CD304 expression; predicted expression data was obtained from Human Protein Atlas.
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871601 25 µg 106 CHF
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871602 100 µg 276 CHF
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Description

CD304 (Neuropilin 1) is a membrane receptor that plays a role in establishment of certain neuronal circuits during neurogenesis, as well as in  organogenesis outside of the nervous system. CD304 binds semaphorin 3A and VEGFA. The interaction between CD304 and VEGFA initiates a signaling pathway that is needed for cell migration and axonal guidance in the motor neuron system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 5.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2861111 (BioLegend Cat. No. 871601)
AB_2861111 (BioLegend Cat. No. 871602)

Antigen Details

Structure
CD304 is a 923 amino acid protein with predicted molecular mass of ~103 kD and observed molecular mass at ~ 120 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, extracellular, cytosol, cytoskeleton, endosome

Function
CD304 is involved in signaling that is needed for cell migration and axonal guidance.
Ligand/Receptor
Semaphorin 3A, VEGFA
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Molecular Family
Semaphorin Receptors
Antigen References
  1. Niland S, Eble JA. 2019. Int. J. Mol. Sci. 20(3).
  2. Gudapati P, et al. 2020. Cytometry B. Clin. Cytom. 10.1002/cyto.b.21866.
  3. Ding Z, et al. 2019. Mol. Carcinog. 58:1019.
Gene ID
8829 View all products for this Gene ID
UniProt
View information about CD304 on UniProt.org
Go To Top Version: 1    Revision Date: 05.06.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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