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Purified anti-CD340 (erbB2/HER-2) Phospho (Tyr1248) Antibody

Pricing & Availability
Clone
A22018C (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine-protein kinase erbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185erbB2, cluster of differentiation 340 (CD340), NGL, VSCN2, TKR1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A22018C_PURE_CD340_Antibody_062123
Whole cell extracts (15 µg total protein) from MCF-7 cells untreated (-) or treated with 100 ng Neuregulin (+) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1248) (clone A22018C) for two hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A22018C_PURE_CD340_Antibody_062123
    Whole cell extracts (15 µg total protein) from MCF-7 cells untreated (-) or treated with 100 ng Neuregulin (+) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1248) (clone A22018C) for two hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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619451 25 µg 170 CHF
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619452 100 µg 425 CHF
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Description

CD340, also commonly known as ErbB2 or HER2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 is involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of CD340 in breast cancers has been shown to confer Taxol resistance, and CD340 is a therapeutic target in a number of human cancers. CD340 activation is accompanied by autophosphorylation at multiple tyrosine residues including Tyr1248.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human CD340 peptide phosphorylated at Tyr1248
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

A22018C is not suitable for ICC or ICFC

RRID
AB_3068143 (BioLegend Cat. No. 619451)
AB_3068143 (BioLegend Cat. No. 619452)

Antigen Details

Structure
CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kD.
Distribution

Plasma membrane, cytoplasm, nucleus

Function
Proto-oncogene, protein tyrosine kinase receptor
Interaction
CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Cell Type
Leukemia
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules, Phospho-Proteins
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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