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Purified anti-cdc2 (p34) Antibody

Pricing & Availability
Clone
POH-1 (See other available formats)
Regulatory Status
RUO
Other Names
Cell division cycle 2, G1 to S and G2 to M, cyclin dependent kinase 1, p34 kinase, CDK1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
POH-1_Pure_cdc2_Antibody_1_071718
Total cell lysates (15 µg protein) from A431 (lane 1), A431 (lane 2), A431 (lane 3), Molt-4 (lane 4) and HeLa (lane 5) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) (lane 1), 1 µg/mL (1:500 dilution) (lane 2) and 2 µg/mL (1:250 dilution) (lane 3, 4, 5) Purified anti-cdc2 (p34) antibody, clone POH-1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-cdc2 (p34) antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: molecular weight ladder.
  • POH-1_Pure_cdc2_Antibody_1_071718
    Total cell lysates (15 µg protein) from A431 (lane 1), A431 (lane 2), A431 (lane 3), Molt-4 (lane 4) and HeLa (lane 5) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) (lane 1), 1 µg/mL (1:500 dilution) (lane 2) and 2 µg/mL (1:250 dilution) (lane 3, 4, 5) Purified anti-cdc2 (p34) antibody, clone POH-1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-cdc2 (p34) antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: molecular weight ladder.
  • POH-1_Pure_cdc2_Antibody_2_071718
    Hela cells stained with purified mouse monoclonal antibody against Cdc2(p34) (clone POH-1), followed by Rhodamine Red-X conjugated goat anti-mouse IgG and DAPI
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626901 25 µg 101 CHF
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Description

Cdc2 (p34), also known as cell division cycle 2, G1 to S and G2 to M; cyclin dependent kinase 1, p34 kinase, and CDK1, is serine/threonine kinase that is ubiquitously expressed in eukaryotes. Cdc2 is found as both a cytoplasmic and nuclear protein. cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally in eukaryotes. The cdc2 protein is highly phosphorylated on threonine, serine, and tyrosine; tyrosine phosphorylation is subject to cell cycle regulation. Cdc2 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. The POH-1 antibody recognizes human, monkey and bovine cdc2 and is useful for Western blotting. The POH-1 antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (formalin-fixed, paraffin embedded), and immunofluorescence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human cdc2 protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
This antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not react with Mouse, Rat, Xenopus, Drosophila.

Additional reported applications (for the relevant formats) include: immunohistochemistry1 of formalin-fixed, paraffin-embeded tissue sections and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Lukas J, et al. 1992. Eur. J. Biochem. 207:169.
Product Citations
  1. Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed
  2. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  3. Liu Y, et al. 2014. J Control Release. 192:114. PubMed
  4. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
RRID
AB_2074779 (BioLegend Cat. No. 626901)

Antigen Details

Structure
Serine/threonine kinase; molecular weight approximately 34 kD
Distribution

Ubiquitous expression in eukaryotes, cytoplasmic and nuclear.

Function
cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally among eukaryotes.
Interaction
Interacts with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, p53
Modification
Highly phosphorylated (threonine, serine, tyrosine); tyrosine phosphorylation subject to cell cycle regulation
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Draetta G, et al. 1988. Nature 336:738.

Gene ID
983 View all products for this Gene ID
UniProt
View information about cdc2 p34 on UniProt.org
Go To Top Version: 2    Revision Date: 06.20.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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