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Purified anti-CDKN2A (p16) Antibody

Pricing & Availability
Clone
15C10C30 (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A (CDK4I), Multiple tumor suppressor 1 (MTS-1), p16-INK4a, p14 ARF
Isotype
Mouse IgG2b, κ
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Product Citations
publications
15C10C30_Purified_CDKN2A_Antibody_1_WB_040115_updated
Total lysates (15µg protein) from 293T and 293T/CDKN2A knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-CDKN2A (p16) antibody, clone 15C10C30 (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-CDKN2A (p16) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 15C10C30_Purified_CDKN2A_Antibody_1_WB_040115_updated
    Total lysates (15µg protein) from 293T and 293T/CDKN2A knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-CDKN2A (p16) antibody, clone 15C10C30 (upper) or 1:3000 diluted anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-CDKN2A (p16) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for GAPDH. Lane M: Molecular weight ladder, M* indicates longer exposure.
  • 15C10C30_Purified_CDKN2A_Antibody_2_IF_040115
    HeLa cells were stained with purified anti-CDKN2A (clone 15C10C30) antibody, followed by staining with DyLight 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor 594® conjugated phalloidin (red).
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675602 100 µg 311 CHF
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Description

Cyclin-dependent kinase inhibitor 2A (CDKN2A) isoform 1, also known as p16-INK4a, is an inhibitor of the cyclin dependent kinases CDK4 and CDK6. CDKN2A acts as a negative regulator of cell cycle progression by preventing phosphorylation of the retinoblastoma protein (pRb) by cyclin-dependent kinases. CDKN2A expression is upregulated during cellular senescence and aging. It functions as a tumor suppressor to induce cell cycle arrest and aging of normal tissues. Germ line point mutations, homozygous deletion, or promoter methylation in the human CDKN2A locus causes uncontrolled cell proliferation, which results in a variety of malignancies such as melanoma, pancreatic adenocarcinoma, gastric cancer, and esophageal cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human CDKN2A recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Milardi G, et al. 2022. Eur J Immunol. 52:1171. PubMed
  2. Pariury H, et al. 2023. Haematologica. :. PubMed
  3. Puca AA, et al. 2022. Cells. 11:. PubMed
  4. Diaz-Flores E, et al. 2019. Cancer Res. 79:2339. PubMed
RRID
AB_2565605 (BioLegend Cat. No. 675602)

Antigen Details

Structure
156 amino acids with a predicted molecular weight of 16.5 kD. It contains 4 ANK repeats that are responsible for protein-protein interaction.
Distribution

Cytoplasm and nucleus.

Function
CDKN2A is an inhibitor of the cyclin dependent kinases CDK4 and CDK6, which plays an imporant role in controlling cell cycle progression from the G1 phase to S phase.
Interaction
CDKN2A forms a heterodimer with CDK4 or CDK6.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Di Cello F, et al. 2013. Leuk. Lymphoma. 54:1762.
2. Shima K, et al. 2011. Int. J. Cancer 128:1080.
3. Baker DJ, et al. 2008. Cell Cycle 7:2795.
4. Jones R, et al. 2007. Cancer Res. 67:9134.
5. Ladanyi M. 2005. Lung Cancer 49:S95.
6. Foulkes WD, et al. 1997. Mol. Med. 3:5.

Gene ID
1029 View all products for this Gene ID
UniProt
View information about CDKN2A on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 01.13.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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