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Purified anti-complement C3/C3b/iC3b/C3d Antibody

Pricing & Availability
Clone
1H8/C3b (See other available formats)
Regulatory Status
RUO
Other Names
Complement Component 3, C3, C3b, iC3b, C3bi, C3d
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1H8slashC3b_PURE_complement_Antibody_1_WB_031016
Western blot of purified anti-complement C3/C3b/iC3b/C3d antibody (clone 1H8/C3b). Lane 1: Molecular weight marker; Lane 2: C3 protein; Lane 3: C3b protein; Lane 4: C3bi protein; Lane 5: Human liver lysate; Lane 6: BSA negative control; Lane 7: Human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1H8slashC3b_PURE_complement_Antibody_1_WB_031016
    Western blot of purified anti-complement C3/C3b/iC3b/C3d antibody (clone 1H8/C3b). Lane 1: Molecular weight marker; Lane 2: C3 protein; Lane 3: C3b protein; Lane 4: C3bi protein; Lane 5: Human liver lysate; Lane 6: BSA negative control; Lane 7: Human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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846302 100 µg 265 CHF
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Description

Complement component 3 (C3) is a 185 kD glycoprotein composed of two chains linked by a disulfide bond. It is the fourth complement component to react in the classical pathway and is a key protein in the alternative and the lectin complement activation pathways. C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35). Opsonization of target surfaces with C3b is central to all three pathways of complement activation. The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H. A ~2 kD fragment of C3b, C3f, is generated when C3b is cleaved at two positions by factor I. Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b/CD18) can expand target-specific B and T cells. iC3b can be cleaved to form C3c and C3dg. Further proteolytic cleavage of C3dg generates C3d and C3g.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human iC3b coupled to sepharose 4B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA - Verified
FC, ICC, ELISA Detection - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry3, immunofluorescent staining2, and ELISA Detection1.

This antibody recognizes human C3, C3b, iC3b, and C3d.

Application References

(PubMed link indicates BioLegend citation)
  1. Tosic L, et al. 1989. J. Immunol. Methods 120:241. (ELISA)
  2. Kennedy AD, et al. 2003. Blood 101:1071. (IF)
  3. Lindorfer MA, et al. 2010. Blood 115:2283. (FC)
Product Citations
  1. Leng K, et al. 2022. Nat Neurosci. 25:1528. PubMed
  2. Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed
  3. Kim H, et al. 2022. Nat Commun. 13:6581. PubMed
RRID
AB_2572128 (BioLegend Cat. No. 846302)

Antigen Details

Structure
C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins. C3d is a 33.8 kD single chain proteolytic fragment of iC3b.
Distribution

Extracellular, plasma membrane, and serum.

Function
C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b and C3d are less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
Interaction
Immune cells interact with C3b, iC3b and C3d using CR1, CR2/CR3 and CR2, respectively.
Ligand/Receptor
C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b/CD18). C3d binds to CR2.
Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.
2. Dempsey PW, et al. 1996. Science 271:348-50.

Gene ID
718 View all products for this Gene ID
UniProt
View information about C3/C3b/iC3b/C3d on UniProt.org

Other Formats

View All C3/C3b/iC3b/C3d Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-complement C3/C3b/iC3b/C3d 1H8/C3b WB,Direct ELISA,FC,ICC,ELISA Detection
Go To Top Version: 2    Revision Date: 05.15.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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