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- Clone
- BL28583 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cystatin B, CSTB, Stefin B, CST6, STFB
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-CSTB antibody (BL28583). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 858301 | 25 µg | 106 CHF | ||||
Cystatin B (CSTB) is a type 1 cystatin belonging to the cystatin superfamily. There are three cystatin subfamilies including type 1 cystatins, type 2 cystatins, and kininogens. Cystatins are thought to have a protective role against the proteases that leak from the lysosomes by inhibiting their cysteine proteinase activity. CSTB can covalently form dimers, tetramers, or oligomers. CSTB oligomers bind to aggregated amyloid-beta (Aβ) and reduce Aβ fibril formation.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant rat CSTB (amino acid sequence Met2~Phe98)
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814580 (BioLegend Cat. No. 858301)
Antigen Details
- Structure
- CSTB is a 98 amino acid protein with predicted molecular mass of ~11 kD and observed molecular mass of ~14 kD.
- Distribution
-
Tissue Distribution: Predominantly expressed in the tonsil, oral mucosa, and bladder
Cellular Distribution: Cytosol, nucleus, and extracellular - Function
- CSTB protects cells from oxidative stress and injury from lysosomal protease leakage.
- Interaction
- CSTB is an inhibitor of cysteine proteinases such as papain and cathepsin B, cathepsin H, and cathepsin L.
- Cell Type
- Microglia, Neurons
- Biology Area
- Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- APP/Aβ Degradation, Enzymes and Regulators, Proteases
- Antigen References
-
- Skerget K, et al. 2010. J Biol Chem. 29:285.
- Tegelberg S, et al. 2012. J Neuropathol Exp Neurol. 71:40.
- Žerovnik E, et al. 2019. Cells. 18:8.
- Gene ID
- 25308 View all products for this Gene ID
- UniProt
- View information about CSTB on UniProt.org
Other Formats
View All CSTB Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-CSTB | BL28583 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-CSTB
Western blot of purified anti-CSTB antibody (BL28583). Lane ...
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