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Purified anti-Cyclin B1 Antibody

Pricing & Availability
Clone
V152 (See other available formats)
Regulatory Status
RUO
Other Names
CCNB1
Isotype
Mouse IgG1
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Product Citations
publications
V152_Pure_Cyclin-B1_Antibody_1_092418
Total cell lysates (15 µg total protein) from HEL, HeLa, A549, Jurkat, and NIH/3T3 cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Cyclin B1 Antibody, clone V152, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • V152_Pure_Cyclin-B1_Antibody_1_092418
    Total cell lysates (15 µg total protein) from HEL, HeLa, A549, Jurkat, and NIH/3T3 cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Cyclin B1 Antibody, clone V152, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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647901 25 µg 124 CHF
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647902 100 µg 265 CHF
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Description

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hamster Cyclin B1
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2.5 - 5.0 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.

Application References

(PubMed link indicates BioLegend citation)
  1. Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed.
  2. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed
  3. Matsumura F, et al. 2011. J. Biol Chem. PubMed
Product Citations
  1. Brazzo JA, et al. 2021. J Cell Sci. 134:. PubMed
  2. Matsumura F, et al. 2011. J Biol Chem. 286:10825. PubMed
  3. Neumann-Staubitz P, et al. 2022. Front Chem. 10:905794. PubMed
  4. Iii JAB, et al. 2021. J Cell Sci. . PubMed
RRID
AB_2072274 (BioLegend Cat. No. 647901)
AB_2072274 (BioLegend Cat. No. 647902)

Antigen Details

Structure
Belongs to the cyclin family. Cyclin AB subfamily.
Distribution

Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.

Interaction
CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Morgan DO, et al.1995. Nature 374:131

Gene ID
891 View all products for this Gene ID
UniProt
View information about Cyclin B1 on UniProt.org
Go To Top Version: 4    Revision Date: 09.01.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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