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Purified anti-Cyclin D1 Antibody

Pricing & Availability
Clone
DCS-6 (See other available formats)
Regulatory Status
RUO
Other Names
B-Cell Lymphoma 1 Protein, B-Cell CLL/Lymphoma 1, PRAD1 Oncogene, G1/S-Specific Cyclin D1, D11S287E, U21B31, PRAD1, BCL1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
DCS-6_PURE_Cyclin_D1_antibody_WB_102615
Western blot analysis of cell lysates from HeLa (lane 1), MCF-7 (lane 2), and NIH3T3 (lane 3). Anti-Cyclin D1 antibody (clone DCS-6) was used, followed by HRP anti-mouse IgG antibody. GAPDH (clone poly6314) was used as loading control.
  • DCS-6_PURE_Cyclin_D1_antibody_WB_102615
    Western blot analysis of cell lysates from HeLa (lane 1), MCF-7 (lane 2), and NIH3T3 (lane 3). Anti-Cyclin D1 antibody (clone DCS-6) was used, followed by HRP anti-mouse IgG antibody. GAPDH (clone poly6314) was used as loading control.
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681902 100 µg 268 CHF
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Description

Cyclin D1, which forms a complex with CDK4 and CDK6, plays central roles in cell cycle transition from G1 to S phase. It also participates in the induction of cellular migration and invasion, enhancement of angiogenesis, inhibition of mitochondrial metabolism, regulation of transcription factor signaling via DNA binding, induction of chromosomal instability, enhancement of DNA damage sensing, and DNA damage repair. The activation of the cyclin D1 oncogene is a major driver of multiple types of human tumors including breast and squamous cell cancers, B-cell lymphoma, myeloma, and parathyroid adenoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human Cyclin D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Garcia Lozano MC, et al. 2006. An. Otorrinolaringol Ibero Am. 33:257.
  2. Lonardo F, et al. 1999. Cancer Res. 10:2470-6.
  3. Pignataro L, et al. 1998. Anticancer Res. 18:1287.
Product Citations
  1. Kozono S, et al. 2018. Nat Commun. 2.50625. PubMed
  2. Demiroglu-Zergeroglu A, et al. 2020. Cell Biol Int. 44:1934. PubMed
RRID
AB_2566566 (BioLegend Cat. No. 681902)

Antigen Details

Structure
295 amino acids with a predicted molecular weight of approximately 34 kD.
Distribution

Nucleus and cytosol.

Function
Cyclin D1 plays central roles in cell cycle transition from G1 to S phase.
Interaction
FBXO4, CDK4, CDK6, CCND1/CDK4/CDKN1B, CDKN1B, UHRF2, USP2, INSM1, the interaction competes with the binding of CCND1 to CDK4 during cell cycle progression and inhibits CDK4 activity.
Biology Area
Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Mitochondrial Function, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Garcia Lozano MC, et al. 2006. An. Otorrinolaringol Ibero Am. 33:257.
2. Lonardo F, et al. 1999. Cancer Res. 59:2470-6.
3. Pignataro L, et al. 1998. Anticancer Res. 18:1287.
4. Darzynkiewicz Z, et al. 2015. Oncotarget. 6:11735.
5. Lamba Saini M, et al. 2015. Diagn. Pathol. 10:32.
6. Casimiro MC, et al. 2014. Expert Opin. Investig. Drugs 23:295.
7. Pestell RG. 2013. Am. J. Pathol. 183:3.

Gene ID
595 View all products for this Gene ID
UniProt
View information about Cyclin D1 on UniProt.org

Related FAQs

There are no FAQs for this product.

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Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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