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Purified anti-Cystatin C Antibody

Pricing & Availability
Clone
E4C6(2A10H7) (See other available formats)
Regulatory Status
RUO
Other Names
Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3
Isotype
Mouse IgG1
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Product Citations
publications
E4C6_2A10H7_PURE_CystatinC_Antibody_WB_072216
Recombinant Human Cystatin C (50 ng) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-Cystatin C antibody (clone E4C6(2A10H7)). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • E4C6_2A10H7_PURE_CystatinC_Antibody_WB_072216
    Recombinant Human Cystatin C (50 ng) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-Cystatin C antibody (clone E4C6(2A10H7)). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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667204 100 µg 323 CHF
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Description

Cystatin C was initially identified in human urine and was named gamma-trace. It is a type II cystatin sub-family member. The cystatin family contains three subfamilies: Type 1 cystatins, also known as stefins, which are predominantly present in the cytosol and the nuclei; Type 2 cystatins are mainly secreted proteins; Type 3 cystatins are multi-domain proteins of high molecular weight (60-120 kD) and are also known as kininogens. Cystatin C inhibits cysteine proteases from the papain family and legumain. It forms tight non-covalent associations with its target enzymes. Cystatin C antagonizes TGF-β signaling in normal and cancer cells in vitro. In this process, Cystatin C binds to the TGF-β type II receptor and inhibits the binding of TGF-β. Cystatin C influences the progression of diseases such as, cancer metastasis, atherosclerosis, aortic aneurisms, emphysema, and arthritis. Also, it is associated with neurodegenerative disease and cerebral angiopathy. Human Cystatin C has the tendency to dimerize in order to produce very tight two-fold symmetric dimers. This amyloidogenic property suggests a mechanism for its aggregation in the brain arteries of elderly people with amyloid angiopathy. The L68Q mutant of human Cystatin C is responsible for conformational disease, which causes massive amyloidosis, cerebral hemorrhage, and death in young adults.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Endogenous protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629541 (BioLegend Cat. No. 667204)

Antigen Details

Structure
Cystatin C is a 13 kD protein which inhibits cystein proteases.
Distribution
Considered ubiquitously expressed, biological fluids, dendritic cells, and macrophages.
Biology Area
Cancer Biomarkers, Cell Biology
Antigen References

1. Grubb A, et al. 1982. Proc. Nat. Acad. Sci. 79:3024.
2. Janowski R, et al. 2001. Nat. Struct. Biol. 8:316.
3. Sokol JP, Schiemann WP. 2004. Mol. Cancer Res. 2:183.
4. Bird PI, et al. 2009. Nat Rev Immunol. 9:871.
5. Kaur G, Levy E. 2012. Front Mol. Neurosci. 5:79.
6. Xu Y, et al. 2014. J Biol Chem. 289:9730.
7. Xu Y, et al. 2015. Immunol. Cell Biol. 5:442-51.

Gene ID
1471 View all products for this Gene ID
UniProt
View information about Cystatin C on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07.26.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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