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Purified anti-Cytochrome c Antibody

Pricing & Availability
Clone
6H2.B4 (See other available formats)
Regulatory Status
RUO
Other Names
Cyt c
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_6H2.B4_PURE_CytochromeC_Antibody_1_IP_090916
Immunoprecipitation/Western Blot analysis using purified anti-Cytochrome c antibody (6H2.B4). 800 µg of HeLa cell lysates was tested at protein concentration of 1µg/µl for each sample. Lane 1 was immunoprecipitated with control antibody and lane 2 was immunoprecipitated with anti-Cytochrome c Antibody (6H2.B4). Western blot analysis was performed using anti-Cytochrome c Antibody (7H8.2C12).
  • 1_6H2.B4_PURE_CytochromeC_Antibody_1_IP_090916
    Immunoprecipitation/Western Blot analysis using purified anti-Cytochrome c antibody (6H2.B4). 800 µg of HeLa cell lysates was tested at protein concentration of 1µg/µl for each sample. Lane 1 was immunoprecipitated with control antibody and lane 2 was immunoprecipitated with anti-Cytochrome c Antibody (6H2.B4). Western blot analysis was performed using anti-Cytochrome c Antibody (7H8.2C12).
  • 6H2.B4_PURE_CytochromeC_Antibody_2_IF_022114
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified cytochrome c (clone 6H2.B4) in blocking buffer overnight at 4°C, and followed by DyLight™ 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.
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612301 25 µg 83 CHF
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612302 100 µg 202 CHF
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Description

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat cyt c-OVA
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

IP - Quality tested
ICC - Verified
ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2.0 - 4.0 µg /1 x107 cell equivalents for immunoprecipitation. For Western blot application, the suggested use of this reagent is 0.5 µg per ml (1:1000 dilution). For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
  2. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
  3. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
  4. Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
  5. Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
  6. She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
  7. McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
Product Citations
  1. Cao YG, et al. 2022. Cell Host Microbe. 1295:30. PubMed
  2. Kruppa AJ et al. 2018. Developmental cell. 44(4):484-499 . PubMed
  3. Wang Z, et al. 2020. Theranostics. 5.401388889. PubMed
  4. She P, et al. 2011. Am J Physiol Endocrinol Metab. 301:E49. PubMed
  5. McGuire K, et al. 2011. J Virol. 85:10806. PubMed
  6. Rana PS, et al. 2020. J Cell Sci. 133:00:00. PubMed
  7. Ryan TA, et al. 2020. EMBO J. 39:e102539. PubMed
  8. Ponsford AH, et al. 2020. Autophagy. :1. PubMed
  9. Cserép C et al. 2018. eNeuro. 5(1) pii: ENEURO. PubMed
RRID
AB_315775 (BioLegend Cat. No. 612301)
AB_315775 (BioLegend Cat. No. 612302)

Antigen Details

Structure
Heme binding domain; 15 kD
Distribution

Mitochondrial intermembrane space

Function
Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction
Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References

1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.

Gene ID
1355 View all products for this Gene ID
UniProt
View information about Cytochrome c on UniProt.org
Go To Top Version: 6    Revision Date: 12.11.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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