- Clone
- 7H8.2C12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyt c
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
612503 | 50 µL | 106 CHF | ||||
612504 | 200 µL | 293 CHF |
Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 7H8.2C12 monoclonal antibody recognizes cytochrome-c from most species and has been shown to be useful for Western blotting.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Hamster
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Horse cyt c-OVA
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 3.0 µg/ml (1:150-1:500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
- The 50 µL size can be used for approximately 5 Western blots, and the 200 µL size can be used for approximately 20 Western blots.
-
Application References
(PubMed link indicates BioLegend citation) -
- Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. (WB)
- Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (WB)
- Product Citations
-
- RRID
-
SCR_001134 (BioLegend Cat. No. 612503)
SCR_001134 (BioLegend Cat. No. 612504)
Antigen Details
- Structure
- Heme binding domain; 15 kD
- Distribution
-
Mitochondrial intermembrane space
- Function
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Interaction
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
1. Liu X, et al. 1996. Cell 86:147.
2. Li P, et al. 1997. Cell 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793. - Gene ID
- 54205 View all products for this Gene ID
- UniProt
- View information about Cytochrome c on UniProt.org
Related FAQs
Other Formats
View All Cytochrome c Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Cytochrome c | 7H8.2C12 | WB,ICC |
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