- Clone
- LDK18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Keratin 18, Keratin type I cytoskeletal 18
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
689102 | 100 µg | 218 CHF |
Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues and is expressed at high levels in some tumor tissues. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been linked to cryptogenic cirrhosis. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3γ, 14-3-3ζ, 14-3-3σ, 14-3-3β, 14-3-3η, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of human keratin 18 (CSETNDTKVLRH-COOH).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
IHC-P - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 0.5 µg/ml (1:1000 - 1:4000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemistry1 on formalin-fixed paraffin-embedded tissue (IHC-P).
This clone and BioLegend clone DA-7 displayed similar affinities when tested for western blot applications
-
Application References
(PubMed link indicates BioLegend citation) -
- Hosoya A. et al. 2010. Anat. Rec. (Hoboken) 293:1864. (IHC-P)
- RRID
-
AB_2629745 (BioLegend Cat. No. 689102)
Antigen Details
- Structure
- Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
- Distribution
-
Keratin 18 is widely expressed in normal single layer epithelial tissues (liver, pancreas) and is expressed at high levels in some tumor tissues.
- Function
- Intermediate filament protein involved with the cytoskeleton.
- Interaction
- Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. USA 100:6063.
2. Kulesh DA, Oshima RG. 1989. Genomics 4:339.
3. Steinert PM, Roop DR. 1988. Ann. Rev. Biochem. 57:593.
4. Izawa I, et al. 2000. J. Biol. Chem. 275:34521.
5. Pankov R, et al. 1994. Proc. Natl. Acad. Sci. USA 91:873. - Gene ID
- 3875 View all products for this Gene ID
- UniProt
- View information about Cytokeratin 18 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Cytokeratin 18 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Cytokeratin 18 | LDK18 | WB,ICC,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us