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Purified anti-Cytokeratin 19 Antibody

Pricing & Availability
Clone
A53-B/A2 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin 19, Keratin 19 type 1 cytoskeletal
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A53-BslashA2_PURE_cytokeratin19_Antibody_1_WB_082316
Total cell lysate (15 µg protein) from MCF-7 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with different concentrations of purified anti-Cytokeratin 19 Antibody (clone A53-B/A2). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection
  • A53-BslashA2_PURE_cytokeratin19_Antibody_1_WB_082316
    Total cell lysate (15 µg protein) from MCF-7 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with different concentrations of purified anti-Cytokeratin 19 Antibody (clone A53-B/A2). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection
  • A53-BslashA2_PURE_cytokeratin19_Antibody_2_IF_082316
    MCF-7 cells were stained with anti-Cytokeratin 19 (clone A53-B/A2), followed by Alexa Fluor® 546 secondary antibody and DAPI (nuclei). Images were aquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.
  • A53-BslashA2_PURE_cytokeratin19_Antibody_3_ICC_080819
    MCF-7 cells were stained with purified anti- Cytokeratin 19 (A53-B/A2) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Nuclei were stained with DAPI (blue).
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628502 100 µg 218 CHF
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Description

Cytokeratin 19, also known as keratin 19, is a type I intermediate filament protein with a molecular weight of approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits. Unlike other cytokeratins, cytokeratin 19 lacks a C-terminal non-helical extension. This cytokeratin is widely expressed in the periderm (transient superficial layer enveloping developing epidermis), muscle, intestine, bile duct, esophagus, stomach, and thymus. Cytokeratin 19 can be upregulated by vitamin A and is thought to play a critical role in embryogenesis. Cytokeratin 19 intereacts with the pinin protein and has been shown to be modified by phosphorylation (Ser10, Ser35).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human mammary carcinoma cell line MCF-7
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
IP, IHC-P, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, immunocytochemistry, and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Karsten U, et al. 1985. Eur. J. Cancer Clin. Oncol. 21:733.
  2. Nishikata T,et al. Anticancer Res. 33:2867. PubMed
Product Citations
  1. Haensel D, et al. 2022. Nat Commun. 13:7520. PubMed
  2. McMahon NP, et al. 2023. Cancers (Basel). 15: . PubMed
  3. Burlingame EA, et al. 2021. Cell Rep Methods. 1:. PubMed
  4. Risom T, et al. 2020. J Clin Invest. 130:231. PubMed
  5. Shalabi SF, et al. 2021. Nat Aging. 1:838. PubMed
  6. NISHIKATA T, et al. 2013. Anticancer Res. 33:2867. PubMed
  7. Rohena C, et al. 2013. Biochem Pharmacol. 85:1104. PubMed
  8. Johnson BE, et al. 2022. Cell Rep Med. 3:100525. PubMed
  9. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
RRID
AB_439773 (BioLegend Cat. No. 628502)

Antigen Details

Structure
Type I intermediate filament protein, contains three coiled-coil domains, lacks C-terminal non-helical extension found in other keratins, approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits.
Distribution

Expressed in the periderm (transient superficial layer enveloping developing epidermis), also expressed in muscle, intestine, bile duct, esophagus, stomach, and thymus. Upregulated by vitamin A.

Function
Intermediate filament protein involved with the cytoskeleton. May play a critical role in embryogenesis
Interaction
Interacts with pinin
Modification
Phosphorylation (Ser10, Ser35)
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Bader BL, et al. 1986. EMBO J. 5:1865.
2. Eckert RL. 1988. Proc. Natl. Acad. Sci. 85:1114.
3. Stasiak PC and Lane EB. 1987 Nucleic Acids Res. 15:10058.

Gene ID
3880 View all products for this Gene ID
UniProt
View information about Cytokeratin 19 on UniProt.org
Go To Top Version: 4    Revision Date: 08.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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