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Purified anti-Cytokeratin 7/17 Antibody

Pricing & Availability
Clone
C-46 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin 7/keratin17, Keratin 7/17
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C-46_PURE_Cytokeratin_Antibody_1_WB_060418
Total lysates (15 µg protein) from HaCaT and HeLa were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.25 or 1µg/ml purified anti-Cytokeratin 7/17 antibody, clone C-46. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • C-46_PURE_Cytokeratin_Antibody_1_WB_060418
    Total lysates (15 µg protein) from HaCaT and HeLa were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.25 or 1µg/ml purified anti-Cytokeratin 7/17 antibody, clone C-46. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
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628702 100 µg 218 CHF
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Description

Cytokeratins are proteins that are involved in the cytoskeleton. The cytokeratin proteins are heterotetramers composed of two type I and two type II subunits. Cytokeratin 17 is a type I protein and cytokeratin 7 is a type II intermediate filament protein, each protein contains three coiled-coil domains. Cytokeratin 7 has an approximate molecular weight of 54 kD; cytokeratin 17 has an approximate molecular weight of 46 kD. Cytokeratin 17 is expressed in the basal cells of complex epithelia, but not in stratified or simple epithelia. Cytokeratin 7 is expressed in the epidermis, bronchus, and mesothelium but not in the colon, exocervix or liver. Mutations in cytokeratin 17 have been shown to result in pachyonychia congenital. Cytokeratin 17 has been shown to interact with the EGF receptor. Cytokeratin 7 has been shown to interact with the EGF receptor and heat shock protein 70 kD1A. The C-46 monoclonal antibody recognizes human, bovine and porcine (but not mouse, rat, or rabbit) cytokeratin 7 and cytokeratin 17 and is useful for Western blotting.This antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cytoskeleton preparation derived from Hela cells
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC-P, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1.25 - 5.0 µg antibody per 5 ml (1:500 - 1:2000) antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone C-46 does not react with Mouse, Rat, or Rabbit.

Application References

(PubMed link indicates BioLegend citation)
  1. Vojtesek B, et al. 1990. Neoplasma 37:333.
Product Citations
  1. Mikolajczyk SD, et al. 2011. J Oncol. 252361:2011. PubMed
RRID
AB_439777 (BioLegend Cat. No. 628702)

Antigen Details

Structure
Type I (cytokeratin 17) and type II (cytokeratin 7) intermediate filament protein, contains three coiled-coil domains. Cytokeratins are heterotetramers composed of two type I and two type II keratins. Cytokeratin 7 has an approximate molecular weight of 5
Distribution

Cytokeratin 17 is expressed in the basal cells of complex epithelia, but not in stratified or simple epithelia. Cytokeratin 7 is expressed in the epidermis, bronchus, and mesothelium but not in the colon, exocervix or liver.

Function
Intermediate filament protein involved with the cytoskeleton. Mutations in cytokeratin 17 have been shown to result in pachyonychia congenita
Interaction
Cytokeratin 17 has been shown to interact with the EGF receptor. Cytokeratin 7 has been shown to interact with the EGF receptor and heat shock protein 70 kD1A.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Glass C, et al. 1988. J. Cell Biol. 107:1337.
2. Troyanovsky SM, et al. 1992. Eur. J. Cell Biol. 59:127.
3. Terrinoni A, et al. 2001. J. Invest. Dermatol. 117:1391.

Gene ID
3855 View all products for this Gene ID
UniProt
View information about Cytokeratin 7/17 on UniProt.org
Go To Top Version: 3    Revision Date: 09.20.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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